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Rodent rhabdomyosarcoma : comparison between total choline concentration at H-MRS and [18F]-fluoromethylcholine uptake at PET using accurate methods for collecting data

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Rommel, Denis [UCL] Bol, Anne [UCL] Abarca-Quinones, Jorge [UCL] Peeters, Frank [UCL] Robert, Annie [UCL] Labar, Daniel [UCL] Galant, Christine [UCL] Grégoire, Vincent [UCL] Duprez, Thierry [UCL]

PURPOSE: To compare choline concentration/amount at proton magnetic resonance spectroscopy (H-MRS) and [(18)F]-fluoromethylcholine (FCH) uptake at positron emission tomography (PET) in a tumour animal model. PROCEDURES: Twenty-two rats bearing grafted syngenic rhabdomyosarcoma in both thighs were examined on a 3T MR system and on a small animal PET system. Total choline concentration was measured on proton MR spectra using a so-called ‘best internal fitting’ volume of interest. Choline uptake was expressed as mean and maximum standardized uptake value (SUV and SUVmax, respectively) and as the percent of injected dose (%ID) after tumour delineation on fused PET-MR images. Data sets were displayed on standard scatter plots. RESULTS: Thirty-six tumours were available for analysis. The area under the curve of the 3.2 ppm choline peak ranged from 69 to 476 (mean, 192) in arbitrary units. Mean SUV values ranged from 0.05 to 0.49 (mean, 0.19) and the %ID from 0.05 to 2.28 (mean, 0.54). Scatter plots failed to reveal quantitative relationship between choline concentration and uptake. Empirically data-driven cut-off lines applied to choline amount (choline concentration × tumour volume) versus choline uptake suggested a paradoxically negative relationship. CONCLUSION: Total choline concentration did not correlate with FCH uptake in a tumour experimental model. A negative feedback of high values of total choline amount on cellular FCH uptake seemed to be present.

Document type Article de périodique (Journal article) – Article de recherche – Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2009
Language Anglais
Journal information "Molecular Imaging and Biology" - Vol. 12, no. 4, p. 415-423 (2010)
Peer reviewed yes
Publisher Springer New York LLC ((United States) New York)
issn 1536-1632
e-issn 1860-2002
Publication status Publié
Affiliations UCL - MD/ESP - Ecole de santé publique
UCL - MD/RAIM - Département de radiologie et d'imagerie médicale
UCL - MD/MNOP - Département de morphologie normale et pathologique
UCL - (SLuc) Service de radiologie
UCL - (SLuc) Service d'anatomie pathologique
UCL - (SLuc) Centre du cancer
UCL - (SLuc) Centre de référence pour l'épilepsie réfractaire
MESH Subject Animals ; Choline - analogs & derivatives, diagnostic use ; Disease Models, Animal ; Fluorine Radioisotopes - diagnostic use ; Magnetic Resonance Spectroscopy ; Positron-Emission Tomography - methods ; Rats ; Rhabdomyosarcoma - pathology, radionuclide imaging, surgery
Keywords H-MRS ; Fluoromethylcholine ; PET ; Animal model ; Rodent ; Cancer imaging
Links
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Bibliographic reference Rommel, Denis ; Bol, Anne ; Abarca-Quinones, Jorge ; Peeters, Frank ; Robert, Annie ; et. al. Rodent rhabdomyosarcoma : comparison between total choline concentration at H-MRS and [18F]-fluoromethylcholine uptake at PET using accurate methods for collecting data. In: Molecular Imaging and Biology, Vol. 12, no. 4, p. 415-423 (2010)
Permanent URL http://hdl.handle.net/2078.1/97813