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An 11.4 kb DNA segment on the left arm of yeast chromosome II carries the carboxypeptidase Y sorting gene PEP1, as well as ACH1, FUS3 and a putative ARS.

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Van Dyck, L Purnelle, Bénédicte [UCL] Skala, J. Goffeau, André [UCL]

We report the nucleotide sequence of an 11.4 kb DNA segment from the left arm of Saccharomyces cerevisiae chromosome II. This sequence contains a typical structure of a functional ARS as well as five open reading frames (ORFs) longer than 300 bp. One is PEP1, a gene encoding a transmembrane protein of 1579 amino acids which transits through the secretory pathway and is involved in vacuolar protein sorting. Two genes were previously sequenced: ACH1 (Lee et al., 1990) and FUS3 (Elion et al., 1990), which encode an acetyl-CoA hydrolase and a protein kinase involved in the cell division cycle, respectively. The last two ORFs localized on the complementary strand of ACH1 are not likely to be expressed.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1992
Journal information "Yeast (Chichester, England)" - Vol. 8, no. 9, p. 769-76 (1992)
Peer reviewed yes
issn 0749-503X
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Amino Acid Sequence ; Base Sequence ; Carboxypeptidases - genetics - metabolism ; Chromosomes, Fungal ; DNA, Fungal - genetics ; Fungal Proteins - genetics - metabolism ; Genes, Fungal ; Molecular Sequence Data ; Open Reading Frames ; Restriction Mapping ; Saccharomyces cerevisiae - genetics - metabolism ; Saccharomyces cerevisiae Proteins ; Vacuoles - metabolism
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Bibliographic reference Van Dyck, L ; Purnelle, Bénédicte ; Skala, J. ; Goffeau, André. An 11.4 kb DNA segment on the left arm of yeast chromosome II carries the carboxypeptidase Y sorting gene PEP1, as well as ACH1, FUS3 and a putative ARS.. In: Yeast (Chichester, England), Vol. 8, no. 9, p. 769-76 (1992)
Permanent URL http://hdl.handle.net/2078.1/9623