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IS231D, E and F, three new insertion sequences in Bacillus thuringiensis : extension of the IS231 family

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Rezsohazy, René [UCL] Hallet, Bernard [UCL] Delcour, Jean [UCL]

IS231 constitutes a family of insertion sequences widespread among Bacillus thuringiensis subspecies. Three new IS231 variants have been isolated from B. thuringiensis subspecies finitimus (IS231 D and E) and israelensis (IS231F). Like the previously described IS231A, B and C, these 1.7 kb elements display single open reading frames encoding 477/478-amino-acid proteins which share between 72% and 88% identity with those of the other members of the family. Sequence comparisons also reveal that all the iso-IS231 terminal inverted repeats are strongly conserved 20 bp sequences. A region susceptible to forming a stable hairpin structure is found just upstream of the open reading frame. Nucleotide substitutions occurring on one strand of the hairpin stems are compensated for by complementary changes at facing positions, giving credence to the hypothesis that this secondary structure plays a role in the regulation of transposition. Examination of IS231 D, E and F flanking sequences reveals that IS231F is bordered by a 12 bp direct repeat. No direct repeats were found flanking IS231D or IS231E.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1992
Journal information "Molecular Microbiology" - Vol. 6, no. 14, p. 1959-67 (1992)
Peer reviewed yes
Publisher Wiley-Blackwell Publishing Ltd. (Chichester)
issn 0950-382X
e-issn 1365-2958
Publication status Publié
Affiliation UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
MESH Subject Amino Acid Sequence ; Bacillus thuringiensis - genetics ; Base Sequence ; DNA Transposable Elements - genetics ; DNA, Bacterial - genetics ; Molecular Sequence Data ; Open Reading Frames - genetics ; Sequence Homology, Nucleic Acid
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Bibliographic reference Rezsohazy, René ; Hallet, Bernard ; Delcour, Jean. IS231D, E and F, three new insertion sequences in Bacillus thuringiensis : extension of the IS231 family. In: Molecular Microbiology, Vol. 6, no. 14, p. 1959-67 (1992)
Permanent URL http://hdl.handle.net/2078.1/9584