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Basolateral membrane conductance in A6 cells: effect of high sodium transport rate.

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Granitzer, M. Nagel, W. Crabbé, J

Conductance of apical and basolateral membranes in short-circuited cultured renal distal cells (A6) was determined using microelectrodes. Epithelia were pre-incubated with 0.1 mumol/l dexamethasone in the presence of 4 mumol/l amiloride to prevent increase in apical Na+ entry. Omission of amiloride increased the Isc from 5.7 to 27.6 microA/cm2 due to the rise in apical membrane conductance from 21 to 595 microS/cm2. Apical fractional resistance decreased from 0.89 to 0.40 and cells depolarized from -52 to -4 mV. Basolateral membrane conductance, which was 320 microS/cm2 at partially inhibited transport, was not significantly altered during the first 2 min following establishment of high transport activity; it started to increase thereafter reaching a more than threefold higher value of 1324 microS/cm2 within 12 min. The gain cannot be explained by increase in partial K+ conductance. Disappearance of the conductance after reduction of basolateral Cl- or in the presence of the Cl- channel blocker 5-nitro-2-(3-phenylpropylamino)benzoate indicates a Cl- conductance, which appears to be activated by depolarization.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1992
Journal information "Pflügers Archiv : European journal of physiology" - Vol. 420, no. 5-6, p. 559-65 (1992)
Peer reviewed yes
issn 0031-6768
Publication status Publié
Affiliation UCL
MESH Subject Amiloride - pharmacology ; Animals ; Biological Transport ; Cell Line ; Cell Membrane - metabolism ; Chloride Channels ; Dexamethasone - pharmacology ; Electric Conductivity - drug effects - physiology ; Kidney Tubules, Distal - cytology - metabolism ; Membrane Proteins - metabolism ; Potassium - metabolism ; Sodium - metabolism
Links
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Bibliographic reference Granitzer, M. ; Nagel, W. ; Crabbé, J. Basolateral membrane conductance in A6 cells: effect of high sodium transport rate.. In: Pflügers Archiv : European journal of physiology, Vol. 420, no. 5-6, p. 559-65 (1992)
Permanent URL http://hdl.handle.net/2078.1/9580