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Mitochondrial and cytosolic expression of human peroxiredoxin 5 in Saccharomyces cerevisiae protect yeast cells from oxidative stress induced by paraquat.

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Tiên Nguyên-nhu, Nhu Knoops, Bernard [UCL]

Human peroxiredoxin 5 is a recently discovered mitochondrial, peroxisomal and cytosolic thioredoxin peroxidase able to reduce hydrogen peroxide and alkyl hydroperoxides. To gain insight into peroxiredoxin 5 antioxidant role in cell protection, we investigated the resistance of yeast cells expressing human peroxiredoxin 5 in mitochondria or in the cytosol against oxidative stress induced by paraquat. The herbicide paraquat is a redox active drug known to generate superoxide anions in mitochondria and the cytosol of yeast and mammalian cells leading to the formation of several reactive oxygen species. Here, we report that mitochondrial and cytosolic human peroxiredoxin 5 protect yeast cells from cytotoxicity and lipid peroxidation induced by paraquat.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2003
Journal information "FEBS letters" - Vol. 544, no. 1-3, p. 148-52 (2003)
Peer reviewed yes
issn 0014-5793
Publication status Publié
Affiliation UCL - SC/BIOL - Département de biologie
MESH Subject Blotting, Western ; Cytosol - metabolism ; DNA, Complementary - metabolism ; Dose-Response Relationship, Drug ; Genetic Vectors ; Herbicides - pharmacology ; Humans ; Lipid Peroxidation ; Microscopy, Fluorescence ; Mitochondria - metabolism ; Models, Genetic ; Oxidation-Reduction ; Oxidative Stress ; Paraquat - pharmacology ; Peroxidases - biosynthesis ; Peroxiredoxins ; Phenotype ; Plasmids - metabolism ; Saccharomyces cerevisiae - metabolism
Links
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Bibliographic reference Tiên Nguyên-nhu, Nhu ; Knoops, Bernard. Mitochondrial and cytosolic expression of human peroxiredoxin 5 in Saccharomyces cerevisiae protect yeast cells from oxidative stress induced by paraquat.. In: FEBS letters, Vol. 544, no. 1-3, p. 148-52 (2003)
Permanent URL http://hdl.handle.net/2078.1/9480