Delvaux, Marc
Demoustier-Champagne, Sophie
[UCL]
Arrays of nanoscopic gold tubes were prepared by electroless deposition of the metal within the pores of polycarbonate particle track-etched membranes (PTM). Glucose oxidase (Gox) was immobilised onto preformed self-assembled monolayers (SAMs) (mercaptoethylamine or mercaptopropionic acid) on electroless gold via cross-linking with glutaraldehyde or covalent attachment by carbodiimide coupling. The effectiveness of the different steps in the Gox immobilisation procedure was assessed by contact angle measurements, cyclic voltammetry and X-ray photoelectron spectroscopy. The enzyme loading was estimated by radioactivity measurements. The sensitivity to beta-glucose of these different biosensors has been evaluated. Glucose responses as large as 400 nA mM(-1) cm(-2) have been obtained. To our knowledge, this sensitivity value is amongst the highest values reported in the literature for comparable biosensor systems.
Bibliographic reference |
Delvaux, Marc ; Demoustier-Champagne, Sophie. Immobilisation of glucose oxidase within metallic nanotubes arrays for application to enzyme biosensors.. In: Biosensors & bioelectronics, Vol. 18, no. 7, p. 943-51 (2003) |
Permanent URL |
http://hdl.handle.net/2078.1/9442 |