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Incorporation of a hydrophobic antibacterial peptide into amphiphilic polyelectrolyte multilayers: A bioinspired approach to prepare biocidal thin coatings

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Document type : Article de périodique (Journal article)
Abstract :

A non-water-soluble natural antibacterial peptide, gramicidin A, has been successfully incorporated into polyelectrolyte assemblies to elaborate biocidal thin films. For this, we used a double strategy, the first step of which consists of complexing the peptide by a non-denaturing anionic amphiphilic polysaccharide, namely a hydrophobically modified carboxymethylpullulan. We demonstrate that the use of this amphiphilic anionic derivative allows to efficiently solubilize the peptide in aqueous solution, without denaturation. The amount of peptide solubilized by the amphiphilic polysaccharide was optimized by systematically varying the hydrophobicity and the molar mass of the CMP derivative. In a second step, the negatively charged complex was layer-by-layer assembled with cationic poly(L-lysine) to form biofunctionalized thin films. The amount of peptide incorporated in the multilayers was controlled by changing the number of deposited complex layers, and was quantified by UV spectroscopy. The antibacterial activity of the resulting biofunctionalized films was I evidenced against a gram-positive bacterium, E. faecalis. We demonstrated that the biocidal activity resulted from a double mechanism: contact between bacteria and the film surface, and release of the peptide into the solution surrounding the film. We also showed that the peptide was not completely removed from the film after rinsing, which insured preservation of the biocidal activity of the film surface.
Access type : Accès restreint
Publication date : 2008
Language : Anglais
Journal information : "Advanced Functional Materials" - Vol. 18, no. 5, p. 758-765 (2008)
Peer reviewed : yes
Publisher : Wiley - V C H Verlag GmbH & Co. KGaA ((Germany) Weinheim)
issn : 1616-301X
e-issn : 1616-3028
Publication status : Publié
Affiliation : UCL - FSA/MAPR - Département des sciences des matériaux et des procédés
Links :
Bibliographic reference Guyomard, Aurelie ; Dé, Emmanuelle ; Jouenne, Thierry ; Malandain, Jean-Jacques ; Muller, Guy ; et. al. Incorporation of a hydrophobic antibacterial peptide into amphiphilic polyelectrolyte multilayers: A bioinspired approach to prepare biocidal thin coatings. In: Advanced Functional Materials, Vol. 18, no. 5, p. 758-765 (2008)
Permanent URL http://hdl.handle.net/2078/83491

References Provided by I4OC

  1. An Yuehuei H., Friedman Richard J., Handbook of Bacterial Adhesion, ISBN:1592592244, 10.1385/1592592244
  2. Katsikogianni, Eur. Cells Mater., 8, 37 (2004)
  3. Costerton, Annu. Rev. Microbiol., 41, 435 (1987)
  4. Donlan, Clin. Microbiol. Rev., 15, 167 (2002)
  5. Hanna, J. Clin. Oncol., 22, 3163 (2004)
  6. Klibanov, J. Mater. Chem., 17, 2479 (2007)
  7. Tiller, Proc. Natl. Acad. Sci. USA, 98, 5981 (2001)
  8. Lee, Biomacromolecules, 5, 877 (2004)
  9. Chung, Int. J. Food Sci. Technol., 38, 165 (2003)
  10. Sunada, J. Photochem. Photobiol. A, 156, 227 (2003)
  11. Ahearn, J. Ind. Microbiol., 15, 372 (1995)
  12. , , , , Thin Solid Films. 2001, 398, 602.
  13. Ignatova, Langmuir, 19, 8971 (2003)
  14. Omae, Appl. Organomet. Chem., 17, 81 (2003)
  15. Soravia, FEBS Lett., 228, 337 (1988)
  16. Zhou, Peptides, 27, 3077 (2006)
  17. Bowie, Protein Pept. Lett., 6, 259 (1999)
  18. Bechinger, Biochim. Biophys. Acta, 1758, 1529 (2006)
  19. Brogden, Nat. Rev. Microbiol., 3, 238 (2005)
  20. Zasloff, Nature, 415, 389 (2002)
  21. Bulet, Immol. Rev., 198, 159 (2004)
  22. Lehrer, Curr. Opin. Immunol., 11, 23 (1999)
  23. Nicolas, Annu. Rev. Microbiol., 49, 277 (1995)
  24. Decher, Thin Solid Films, 210, 831 (1992)
  25. Bertrand, Macromol. Rapid Commun., 21, 319 (2000)
  26. Multilayer Thin Films. Sequential Assembly of Nanocomposite Materials, (Eds: ), Wiley-VCH, Weinheim, Germany 2003.
  27. , , , in Supramolecular Polymers, (Ed: ), Marcel Dekker, New York, NY 2000, p. 505.
  28. Hammond, Curr. Opin. Colloid Interface Sci., 4, 430 (1999)
  29. Schönhoff, Curr. Opin. Colloid Interface Sci., 8, 86 (2003)
  30. Li, Langmuir, 22, 9820 (2006)
  31. Grunlan, Biomacromolecules, 6, 1149 (2005)
  32. Dai, Nano Lett., 2, 497 (2002)
  33. Podsiadlo, Langmuir, 21, 11915 (2005)
  34. Shi, J. Biomed. Mater. Res. Part A, 76, 826 (2006)
  35. Lee, Langmuir, 21, 9651 (2005)
  36. Fu, Biomaterials, 26, 6684 (2005)
  37. Etienne, Biomaterials, 26, 6704 (2005)
  38. Etienne, Antimicrob. Agents Chemother., 48, 3662 (2004)
  39. Rudra, J. Biomater. Sci. Polym. Ed., 17, 1301 (2006)
  40. Guyomard, Langmuir, 22, 2281 (2006)
  41. Duval-Terrié, Protein Sci., 12, 681 (2003)
  42. Picard, Protein Sci., 13, 3056 (2004)
  43. Guyomard, Macromolecules, 38, 5737 (2005)
  44. Hotchkiss, J. Biol. Chem., 132, 793 (1940)
  45. Wooley, J. Membr. Biol., 129, 109 (1992)
  46. Sarges, J. Am. Chem. Soc., 87, 2020 (1965)
  47. Richards, Infect. Control. Hosp. Epidemiol., 21, 510 (2000)
  48. Jagannadham, Biopolymers, 80, 708 (2005)
  49. Duval, Colloids Surf. A, 220, 105 (2003)
  50. Duval, J. Chromatogr. B, 753, 115 (2001)
  51. Ph.D. Thesis, Université de Rouen, France 2002.
  52. Wallace, Biophys. J., 49, 295 (1986)
  53. Isbell, FEBS Lett., 25, 192 (1972)
  54. Veatch, Biochemistry, 13, 5249 (1974)
  55. Chen, Biopolymers, 42, 771 (1997)
  56. Bataille, Int. J. Biol. Macromol., 20, 179 (1997)
  57. Aspnes, Phys. Rev. B, 27, 985 (1998)