User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Expression analysis of two gene subfamilies encoding the plasma membrane H+-ATPase in Nicotiana plumbaginifolia reveals the major transport functions of this enzyme.

Primary tabs

Moriau, L Michelet, B. Bogaerts, Pierre [UCL] Lambert, L. Michel, A. Boutry, Marc [UCL]

The plasma membrane H+-ATPase couples ATP hydrolysis to proton transport, thereby establishing the driving force for solute transport across the plasma membrane. In Nicotiana plumbaginifolia, this enzyme is encoded by at least nine pma (plasma membrane H+-ATPase) genes. Four of these are classified into two gene subfamilies, pma1-2-3 and pma4, which are the most highly expressed in plant species. We have isolated genomic clones for pma2 and pma4. Mapping of their transcript 5' end revealed the presence of a long leader that contained small open reading frames, regulatory features typical of other pma genes. The gusA reporter gene was then used to determine the expression of pma2, pma3 and pma4 in N. tabacum. These data, together with those obtained previously for pma1, led to the following conclusions. (i) The four pma-gusA genes were all expressed in root, stem, leaf and flower organs, but each in a cell-type specific manner. Expression in these organs was confirmed at the protein level, using subfamily-specific antibodies. (ii) pma4-gusA was expressed in many cell types and notably in root hair and epidermis, in companion cells, and in guard cells, indicating that in N. plumbaginifolia the same H+-ATPase isoform might be involved in mineral nutrition, phloem loading and control of stomata aperture. (iii) The second gene subfamily is composed, in N. plumbaginifolia, of a single gene (pma4) with a wide expression pattern and, in Arabidopsis thaliana, of three genes (aha1, aha2, aha3), at least two of them having a more restrictive expression pattern. (iv) Some cell types expressed pma2 and pma4 at the same time, which encode H+-ATPases with different enzymatic properties.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1999
Language Anglais
Journal information "The Plant journal : for cell and molecular biology" - Vol. 19, no. 1, p. 31-41 (1999)
Peer reviewed yes
issn 0960-7412
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Amino Acid Sequence ; Base Sequence ; Cloning, Molecular ; DNA, Plant ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Plant ; Histocytochemistry ; Molecular Sequence Data ; Multigene Family ; Plants, Toxic ; Proton-Translocating ATPases - genetics - metabolism ; RNA, Messenger - genetics ; Tobacco - enzymology
Links
  1. Michelet B., Plant Physiol., 108, 1 (1995)
  2. Palmgren M.G., Adv. Bot. Res., 28, 1 (1998)
  3. Serrano R, Structure And Function Of Plasma Membrane ATPase, 10.1146/annurev.arplant.40.1.61
  4. Sussman M., Molecular Analysis of Proteins in the Plant Plasma Membrane, 10.1146/annurev.arplant.45.1.211
  5. DeWitt N. D., Immunocytological Localization of an Epitope-Tagged Plasma Membrane Proton Pump (H+-ATPase) in Phloem Companion Cells, 10.1105/tpc.7.12.2053
  6. Parets-Soler A., Plant Physiol., 93, 1654 (1990)
  7. Samuels A.L., Plant Physiol., 99, 1509 (1992)
  8. Frommer W.B., J. Exp. Bot., 46, 587 (1995)
  9. Bouche-Pillon S., Planta, 193, 392 (1994)
  10. Becker D., Planta, 190, 44 (1993)
  11. Nakajima N., Plant Cell Physiol., 36, 919 (1995)
  12. Rayle D.L., Plant Physiol., 99, 1271 (1992)
  13. Ewing N. N., Bennett A. B., Assessment of the Number and Expression of P-Type H+-ATPase Genes in Tomato, 10.1104/pp.106.2.547
  14. Harper J.F., Mol. Gen. Genet, 244, 572 (1994)
  15. Perez C., J. Biol. Chem., 267, 1204 (1992)
  16. Moriau L., Plant Mol. Biol., 21, 955 (1993)
  17. Frias I., A Major Isoform of the Maize Plasma Membrane H+-ATPase: Characterization and Induction by Auxin in Coleoptiles, 10.1105/tpc.8.9.1533
  18. Harms K., Plant Mol. Biol., 26, 979 (1994)
  19. Jin Y. K., Integration and Nonrandom Mutation of a Plasma Membrane Proton ATPase Gene Fragment within the Bs1 Retroelement of Maize, 10.1105/tpc.6.8.1177
  20. Michelet B., A Plant Plasma Membrane Proton-ATPase Gene Is Regulated by Development and Environment and Shows Signs of a Translational Regulation, 10.1105/tpc.6.10.1375
  21. Joshi, Nucl. Acids Res., 15, 6643 (1987)
  22. Lukaszewicz M., Plant J., 14, 413 (1998)
  23. d'Exaerde Alban de Kerchove, Supply Philip, Dufour Jean-Pierre, Bogaerts Pierre, Thinès Denyse, Goffeau André, Boutry Marc, Functional Complementation of a Null Mutation of the YeastSaccharomyces cerevisiaePlasma Membrane H+-ATPase by a Plant H+-ATPase Gene, 10.1074/jbc.270.40.23828
  24. Luo Hong, Morsomme Pierre, Boutry Marc, The Two Major Types of Plant Plasma Membrane H+-ATPases Show Different Enzymatic Properties and Confer Differential pH Sensitivity of Yeast Growth, 10.1104/pp.119.2.627
  25. Gunning B.E.S., Protoplasma, 71, 147 (1970)
  26. Kuhn C., Macromolecular Trafficking Indicated by Localization and Turnover of Sucrose Transporters in Enucleate Sieve Elements, 10.1126/science.275.5304.1298
  27. Young J.F., Genetics, 149, 501 (1998)
  28. DeWitt ND, Harper JF, Sussman MR, Evidence for a plasma membrane proton pump in phloem cells of higher plants., 10.1046/j.1365-313x.1991.t01-17-00999.x
  29. Hentzen A. E., Smart L. B., Whimmers L. E., Fang H. H., Schroeder J. I., Bennett A. B., Two Plasma Membrane H+-ATPase Genes Expressed in Guard Cells of Vicia faba Are Also Expressed Throughout the Plant, 10.1093/oxfordjournals.pcp.a028994
  30. Jones J.T., Plant Mol. Biol., 28, 983 (1995)
  31. Boutry M., EMBO J., 4, 2159 (1985)
  32. Jefferson R.A., EMBO J., 6, 3901 (1987)
  33. Horsch R.B., Proc. Natl Acad. Sci. USA, 83, 2571 (1986)
Bibliographic reference Moriau, L ; Michelet, B. ; Bogaerts, Pierre ; Lambert, L. ; Michel, A. ; et. al. Expression analysis of two gene subfamilies encoding the plasma membrane H+-ATPase in Nicotiana plumbaginifolia reveals the major transport functions of this enzyme.. In: The Plant journal : for cell and molecular biology, Vol. 19, no. 1, p. 31-41 (1999)
Permanent URL http://hdl.handle.net/2078.1/8316