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Rabbit sarcoplasmic reticulum Ca(2+)-ATPase replaces yeast PMC1 and PMR1 Ca(2+)-ATPases for cell viability and calcineurin-dependent regulation of calcium tolerance.

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Degand, I Catty, P. Talla, E Thinès-Sempoux, D de Kerchove d'Exaerde, A Goffeau, André [UCL] Ghislain, Michel [UCL]

SERCA1a, the fast-twitch skeletal muscle isoform of sarco(endo)plasmic reticulum Ca(2+)-ATPase, was expressed in yeast using the promoter of the plasma membrane H(+)-ATPase. In the yeast Saccharomyces cerevisiae, the Golgi PMR1 Ca(2+)-ATPase and the vacuole PMC1 Ca(2+)-ATPase function together in Ca2+ sequestration and Ca2+ tolerance. SERCA1a expression restored growth of pmc1 mutants in media containing high Ca2+ concentrations, consistent with increased Ca2+ uptake in an internal compartment. SERCA1a expression also prevented synthetic lethality of pmr1 pmc1 double mutants on standard media. Electron microscopy and subcellular fractionation analysis showed that SERCA1a was localized in intracellular membranes derived from the endoplasmic reticulum. Finally, we found that SERCA1a ATPase activity expressed in yeast was regulated by calcineurin, a Ca2+/calmodulin-dependent phosphoprotein phosphatase. This result indicates that calcineurin contributes to calcium homeostasis by modulating the ATPase activity of Ca2+ pumps localized in intra-cellular compartments.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1999
Language Anglais
Journal information "Molecular microbiology" - Vol. 31, no. 2, p. 545-56 (1999)
Peer reviewed yes
issn 0950-382X
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject ATP-Binding Cassette Transporters - metabolism ; Animals ; Calcineurin - metabolism ; Calcium - metabolism ; Calcium-Transporting ATPases - genetics - metabolism ; Fungal Proteins - metabolism ; Gene Expression ; Intracellular Membranes - metabolism ; Isoenzymes - genetics - metabolism ; Phosphoproteins - metabolism ; Plasma Membrane Calcium-Transporting ATPases ; Rabbits ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae Proteins ; Sarcoplasmic Reticulum - enzymology
Links
  1. Amory A., J Biol Chem, 255, 9353 (1980)
  2. Andersen J.P., Bioscience Reports, 15, 243 (1995)
  3. Antebi A., Fink G. R., The yeast Ca(2+)-ATPase homologue, PMR1, is required for normal Golgi function and localizes in a novel Golgi-like distribution., 10.1091/mbc.3.6.633
  4. Boutry Marc, Foury Françoise, Goffeau André, Energy-dependent uptake of calcium by the yeast Schizosaccharomyces pombe, 10.1016/0005-2736(77)90034-7
  5. Capieaux E., J Biol Chem, 264, 7437 (1989)
  6. Capieaux E., J Biol Chem, 268, 21895 (1993)
  7. Carafoli E, Intracellular Calcium Homeostasis, 10.1146/annurev.biochem.56.1.395
  8. Centeno Francisco, Deschamps Stéphane, Lompré Anne-Marie, Anger Marielle, Moutin Marie-Jo, Dupont Yves, Palmgren Michael Gjedde, Villalba Jose Manuel, Møller Jesper Vuust, Falson Pierre, le Maire Marc, Expression of the sarcoplasmic reticulum Ca2+-ATPase in yeast, 10.1016/0014-5793(94)01104-4
  9. Cunningham K. W., Calcineurin-dependent growth control in Saccharomyces cerevisiae mutants lacking PMC1, a homolog of plasma membrane Ca2+ ATPases, 10.1083/jcb.124.3.351
  10. Cunningham K W, Fink G R, Calcineurin inhibits VCX1-dependent H+/Ca2+ exchange and induces Ca2+ ATPases in Saccharomyces cerevisiae., 10.1128/mcb.16.5.2226
  11. Cyert M S, Thorner J, Regulatory subunit (CNB1 gene product) of yeast Ca2+/calmodulin-dependent phosphoprotein phosphatases is required for adaptation to pheromone., 10.1128/mcb.12.8.3460
  12. Cyert M.S., Proc Natl Acad Sci USA, 88, 7376 (1991)
  13. 13L. De Meis (1981 ) InThe Sarcoplasmic Reticulum-Transport and Energy Transduction,Vol 2. Bittar, E. (ed.). New York: John Wiley Sons, pp. 1 162 .
  14. Dufour J.-P., Methods Enzymol, 157, 528 (1988)
  15. Dupont Y., Eur J Biochem, 72, 185 (1977)
  16. Dupont Yves, Low-temperature studies of the sarcoplasmic reticulum calcium pump, 10.1016/0005-2736(82)90580-6
  17. Durr G., Strayle J., Plemper R., Elbs S., Klee S. K., Catty P., Wolf D. H., Rudolph H. K., The medial-Golgi Ion Pump Pmr1 Supplies the Yeast Secretory Pathway with Ca2+ and Mn2+ Required for Glycosylation, Sorting, and Endoplasmic Reticulum-Associated Protein Degradation, 10.1091/mbc.9.5.1149
  18. Eilam Y., Microbios, 35, 99 (1982)
  19. Enouf J., J Biol Chem, 263, 13922 (1988)
  20. Falson Pierre, Menguy Thierry, Corre Fabienne, Bouneau Laurence, de Gracia Adrienne Gomez, Soulié Stéphanie, Centeno Francisco, Møller Jesper Vuust, Champeil Philippe, le Maire Marc, The Cytoplasmic Loop between Putative Transmembrane Segments 6 and 7 in Sarcoplasmic Reticulum Ca2+-ATPase Binds Ca2+and Is Functionally Important, 10.1074/jbc.272.28.17258
  21. Foletti D., Faseb J, 9, 670 (1995)
  22. Poor Forrest, Parent Stephen A., Morin Nancy, Dahl Arlene M., Ramadan Naasa, Chrebet Gary, Bostian Keith A., Nielsen Jennifer B., Calcineurin mediates inhibition by FK506 and cyclosporin of recovery from α-factor arrest in yeast, 10.1038/360682a0
  23. Foury F., Eur J Biochem, 119, 395 (1981)
  24. Kuo C L, Campbell J L, Cloning of Saccharomyces cerevisiae DNA replication genes: isolation of the CDC8 gene and two genes that compensate for the cdc8-1 mutation., 10.1128/mcb.3.10.1730
  25. Laemmli U.K., Nature, 227, 680 (1970)
  26. Lowry O.H., J Biol Chem, 193, 265 (1951)
  27. MacLennan D.H., Biophys J, 58, 1355 (1990)
  28. Masuda H., Biochemistry, 12, 4581 (1973)
  29. Matheos D.P., Genes Dev, 11, 3445 (1997)
  30. Møller Jesper V, Juul Birte, le Maire Marc, Structural organization, ion transport, and energy transduction of P-type ATPases, 10.1016/0304-4157(95)00017-8
  31. Moutin M.-J., J Biol Chem, 269, 11147 (1994)
  32. Mumberg D., Nucleic Acids Res, 22, 5767 (1994)
  33. Nass Richard, Cunningham Kyle W., Rao Rajini, Intracellular Sequestration of Sodium by a Novel Na+/H+Exchanger in Yeast Is Enhanced by Mutations in the Plasma Membrane H+-ATPase : INSIGHTS INTO MECHANISMS OF SODIUM TOLERANCE, 10.1074/jbc.272.42.26145
  34. Pedersen Peter L., Carafoli Ernesto, Ion motive ATPases. I. Ubiquity, properties, and significance to cell function, 10.1016/0968-0004(87)90071-5
  35. Petithory J.R., Biochemistry, 27, 5553 (1988)
  36. Posas Francesc, Camps Manel, Ario Joaqun, The PPZ Protein Phosphatases Are Important Determinants of Salt Tolerance in Yeast Cells, 10.1074/jbc.270.22.13036
  37. Pozos T C, Sekler I, Cyert M S, The product of HUM1, a novel yeast gene, is required for vacuolar Ca2+/H+ exchange and is related to mammalian Na+/Ca2+ exchangers., 10.1128/mcb.16.7.3730
  38. Reis E.M.R., Biosci Rep, 16, 107 (1996)
  39. Rose M.D., Cell, 57, 1211 (1989)
  40. Rudolph Hans K., Antebi Adam, Fink Gerald R., Buckley Catherine M., Dorman Thomas E., LeVitre JoAnn, Davidow Lance S., Mao Jen-i, Moir Donald T., The yeast secretory pathway is perturbed by mutations in PMR1, a member of a Ca2+ ATPase family, 10.1016/0092-8674(89)90410-8
  41. Sagara Y., J Biol Chem, 266, 13503 (1991)
  42. Serrano R., Nature, 319, 689 (1986)
  43. Sikorski R.S., Genetics, 122, 19 (1989)
  44. Sorin Alexander, Rosas Gisele, Rao Rajini, PMR1, a Ca2+-ATPase in Yeast Golgi, Has Properties Distinct from Sarco/endoplasmic Reticulum and Plasma Membrane Calcium Pumps, 10.1074/jbc.272.15.9895
  45. Stathopoulos A.M., Genes Dev, 11, 3432 (1997)
  46. Supply P., J Biol Chem, 268, 19744 (1993)
  47. Tanida Isei, Hasegawa Akira, Iida Hidetoshi, Ohya Yoshikazu, Anraku Yasuhiro, Cooperation of Calcineurin and Vacuolar H+-ATPase in Intracellular Ca2+Homeostasis of Yeast Cells, 10.1074/jbc.270.17.10113
  48. 48D.A. Treco (1989 ) Basic techniques of yeast genetics. InCurrent Protocols in Molecular Biology. Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (eds). New York: Wiley-Interscience, pp. 13.1.1 13.1.7 .
  49. Van Veldhoven P.P., Anal Biochem, 161, 45 (1987)
  50. Vianna Antonio Luiz, Interaction of calcium and magnesium in activating and inhibiting the nucleoside triphosphatase of sarcoplasmic reticulum vesicles, 10.1016/0005-2744(75)90241-7
  51. Wach Achim, PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae, 10.1002/(sici)1097-0061(19960315)12:3<259::aid-yea901>3.0.co;2-c
  52. Wallis John W., Chrebet Gary, Brodsky Gary, Rolfe Mark, Rothstein Rodney, A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase, 10.1016/0092-8674(89)90855-6
  53. Weber K., J Biol Chem, 244, 4406 (1969)
  54. Winston F., Yeast, 11, 53 (1995)
  55. Withee J.L., Genetics, 149, 865 (1998)
Bibliographic reference Degand, I ; Catty, P. ; Talla, E ; Thinès-Sempoux, D ; de Kerchove d'Exaerde, A ; et. al. Rabbit sarcoplasmic reticulum Ca(2+)-ATPase replaces yeast PMC1 and PMR1 Ca(2+)-ATPases for cell viability and calcineurin-dependent regulation of calcium tolerance.. In: Molecular microbiology, Vol. 31, no. 2, p. 545-56 (1999)
Permanent URL http://hdl.handle.net/2078.1/8236