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Development and validation of stable reference materials for food microbiology using Bacillus cereus and Clostridium perfringens spores.

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Abdel Massih, Marleen [UCL] Planchon, Viviane [CRA-W] Anceau, Christine [ULG] Mahillon, Jacques [UCL]

Aims:  To develop a new type of microbiological Reference Materials (RMs), displaying long-term stability at room temperature. The purpose was to produce and validate two batches of RMs for the enumeration of Bacillus cereus and Clostridium perfringens. Methods and Results:  The RMs were based on spores of B. cereus and Cl. perfringens, adsorbed on calcium carbonate pellets. Two batches of 1000 units were manufactured and validated in compliance with ISO guide 35. After verification of their homogeneity, the stability of the 'RM-B. cereus' and 'RM-Cl. perfringens' batches was proven during at least 36 and 9 months, respectively, at room temperature. The validation study was completed by international collaborative trial involving 12 laboratories, allowing the validation of the assigned values. Conclusions:  The methodology developed in this work enabled to produce easy-to-handle and cost-effective RMs, displaying an unprecedented stability at room temperature, a good homogeneity and a precise and validated assigned value. Significance and Impact of the Study:  This study revealed new paths for the development of stable microbiological RMs. Overcoming the intrinsic instability of the living cells makes it possible to produce valuable tools for the quality assurance of microbiology laboratories.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't, Validation Studies
Access type Accès restreint
Publication date 2011
Language Anglais
Journal information "Journal of applied microbiology" - Vol. 110, no. 6, p. 1524-1530 (2011)
Peer reviewed yes
Publisher A. Gilmour
issn 1364-5072
e-issn 1365-2672
Publication status Publié
Affiliations UCL - SST/ELI/ELIM - Applied Microbiology
CRA-W - Statistique
ULG - GxABT
MESH Subject Temperature ; Spores, Bacterial - growth & development ; Reference Standards ; Food Microbiology - methods - standards ; Colony Count, Microbial ; Clostridium perfringens - growth & development ; Bacteriological Techniques - methods ; Bacillus cereus - growth & development
Keywords Reference Material ; Bacillus ; Clostridium ; Food microbiology
Links
  1. Anonymous, ISO Guide 30: Terms and Definitions Used in Connection with Reference Materials (1992Amd1:2008)
  2. Anonymous, ISO 5725-2: Accuracy (Trueness and Precision) of Measurement Methods and Results - Basic Method for the Determination of Repeatability and Reproducibility of a Standard Measurement Method (1994)
  3. Anonymous, ISO 7932: Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the Enumeration of Presumptive Bacillus cereus - Colony-Count Technique at 30 degrees C (2004a)
  4. Anonymous, ISO 7937: Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the Enumeration of Clostridium perfringens - Colony-Count Technique (2004b)
  5. Anonymous, ISO 13528: Statistical Methods for Use in Proficiency Testing by Interlaboratory Comparisons (2005)
  6. Anonymous, ISO Guide 35: Reference Materials - General and Statistical Principles for Certification (2006)
  7. Anonymous, ISO Guide 34: General Requirements for the Competence of Reference Materials Producers (2009)
  8. Dierick K., Van Coillie E., Swiecicka I., Meyfroidt G., Devlieger H., Meulemans A., Hoedemaekers G., Fourie L., Heyndrickx M., Mahillon J., Fatal Family Outbreak of Bacillus cereus-Associated Food Poisoning, 10.1128/jcm.43.8.4277-4279.2005
  9. GOLDEN NEAL J., CROUCH EDMUND A., LATIMER HEEJEONG, KADRY ABDEL-RAZAK, KAUSE JANELL, Risk Assessment for Clostridium perfringens in Ready-to-Eat and Partially Cooked Meat and Poultry Products, 10.4315/0362-028x-72.7.1376
  10. HOFFMANS CYNTHIA M., FUNG DANIEL Y.C., EFFECTIVE METHOD FOR DRY INOCULATION of BACTERIAL CULTURES, 10.1111/j.1745-4581.1992.tb00275.x
  11. Horneck G., Bücker H., Reitz G., Long-term survival of bacterial spores in space, 10.1016/0273-1177(94)90448-0
  12. Hornstra L. M., de Vries Y. P., de Vos W. M., Abee T., Influence of Sporulation Medium Composition on Transcription of ger Operons and the Germination Response of Spores of Bacillus cereus ATCC 14579, 10.1128/aem.72.5.3746-3749.2006
  13. In't Veld P.H, The use of reference materials in quality assurance programmes in food microbiology laboratories., 10.1016/s0168-1605(98)00145-7
  14. de JONG A. E. I., BEUMER R. R., ROMBOUTS F. M., Optimizing Sporulation of Clostridium perfringens, 10.4315/0362-028x-65.9.1457
  15. Lisanby M. W., Swiecki M. K., Dizon B. L. P., Pflughoeft K. J., Koehler T. M., Kearney J. F., Cathelicidin Administration Protects Mice from Bacillus anthracis Spore Challenge, 10.4049/jimmunol.181.7.4989
  16. Philipp Wolfgang J., van Iwaarden Pierre, Schimmel Heinz, Meeus Nele, Kollmorgen Nadine, Development of reference materials for microbiological analysis, 10.1007/s00769-006-0244-3
  17. Thompson Michael, Ellison Stephen L. R., Wood Roger, The International Harmonized Protocol for the proficiency testing of analytical chemistry laboratories (IUPAC Technical Report), 10.1351/pac200678010145
  18. Vander Heyden Yvan, Smeyers-Verbeke Johanna, Set-up and evaluation of interlaboratory studies, 10.1016/j.chroma.2007.02.053
Bibliographic reference Abdel Massih, Marleen ; Planchon, Viviane ; Anceau, Christine ; Mahillon, Jacques. Development and validation of stable reference materials for food microbiology using Bacillus cereus and Clostridium perfringens spores.. In: Journal of applied microbiology, Vol. 110, no. 6, p. 1524-1530 (2011)
Permanent URL http://hdl.handle.net/2078.1/76081