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Hybridization in annual plants: patterns and dynamics during a four-year study in mixed Rhinanthus populations

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Ducarme, Véronique [UCL] Vrancken, Jérôme [UCL] Wesselingh, Renate A. [UCL]

Hybridization in annual plants is rare, but their short life cycle provides an excellent opportunity to study the dynamics of hybridization. Hybridization occurs between the annual hemiparasites Rhinanthus minor and Rhinanthus angustifolius (Orobanchaceae). Using flower morphology, Kwak (1980) found a prevalence of hybrids close to R. angustifolius in a single population. We aim to find whether this pattern is also found using genetic markers, whether it is generally occurring in mixed populations, and whether these populations are stable over time. We used species-specific genetic markers to determine the number of individuals in a range of hybrid classes in three mixed populations of different ages during four consecutive years. In the young population, F-1 hybrids were found in the first year and mostly hybrids between R. minor and these F(1)s in the second year, but in the years after that, hybrids close to R. angustifolius became more abundant. We also found this in the two older populations, where hybrids close to R. angustifolius always occurred in higher frequencies than hybrids close to R. minor. Over time, R. angustifolius strongly increased in frequency in two populations. Patterns of marker presence and absence suggested that advanced-generation hybrids are mainly formed by backcrossing with one of the parents, predominantly R. angustifolius whenever its frequency in the population is higher than 15%. The dynamics of mixed populations depend on the ecological conditions that regulate the presence of the two parental species, and introgression into R. angustifolius seems prevalent.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2010
Language Anglais
Journal information "Folia Geobotanica" - Vol. 45, no. 4, p. 387-405 (2010)
Peer reviewed yes
Publisher Springer Netherlands ((Netherlands) Dordrecht)
issn 1211-9520
Publication status Publié
Affiliation UCL - SST/ELI/ELIB - Biodiversity
Keywords CpDNA ; Genetic markers ; Hybrid zone ; Introgression ; ISSR ; RAPD ; 3123
Links
  1. Ameloot E, Verheyen K, Bakker JP, de Vries Y, Hermy M (2006) Long-term dynamics of the hemiparasite Rhinanthus angustifolius and its relationship with vegetation structure. J Veg Sci 17:637–646
  2. Anderson Edgar, Introgressive hybridization., 10.5962/bhl.title.4553
  3. Arnold ML (1993) Iris nelsonii (Iridaceae): origin and genetic composition of a homoploid hybrid species. Amer J Bot 80:577–583
  4. Arnold ML (1997) Natural hybridization and evolution. Oxford University Press, New York
  5. Bakker JP (2006) Vegetation conservation, management and restoration. In van der Maarel E (ed) Vegetation ecology. Blackwell Publishing, Oxford, pp 309–331
  6. Barton NH, Hewitt GM (1985) Analysis of hybrid zones. Annual Rev Ecol Syst 16:113–148
  7. Britch SC, Cain ML, Howard DJ (2001) Spatio-temporal dynamics of the Allonemobius fasciatus-A. socius mosaic hybrid zone: a 14-year perspective. Molec Ecol 10:627–638
  8. Buerkle CA (2005) Maximum-likelihood estimation of a hybrid index based on molecular markers. Molec Ecol Notes 5:684–687
  9. Burgess KS, Morgan M, Deverno L, Husband BC (2005) Asymmetrical introgression between two Morus species (M. alba, M. rubra) that differ in abundance. Molec Ecol 14:3471–3483
  10. Burke JM, Voss TJ, Arnold ML (1998) Genetic interactions and natural selection in Louisiana iris hybrids. Evolution 52:1304–1310
  11. Campion-Bourget F (1980a) L’autogamie et l’allogamie chez les cinq principales espèces de Rhinanthus L. de France. Rev Cytol Biol Veg Bot 3:199–219
  12. Campion-Bourget F (1980b) L’hybridation interspécifique expérimentale chez les cinq principales espèces de Rhinanthus L. de France. Rev Cytol Biol Veg Bot 3:221–280
  13. Carney Shanna E., Gardner Keith A., Rieseberg Loren H., EVOLUTIONARY CHANGES OVER THE FIFTY-YEAR HISTORY OF A HYBRID POPULATION OF SUNFLOWERS (HELIANTHUS), 10.1111/j.0014-3820.2000.tb00049.x
  14. Cattell MV, Karl SA (2004) Genetics and morphology in a Borrichia frutescens and B. arborescens (Asteraceae) hybrid zone. Amer J Bot 91:1757–1766
  15. Cruzan MB, Arnold ML (1993) Ecological and genetic associations in an Iris hybrid zone. Evolution 47:1432–1445
  16. De Hullu Ella, Brouwer Tineke, Ter Borg S. J., ANALYSIS OF THE DEMOGRAPHY OF RHINANTHUS ANGUSTIFOLIUS POPULATIONS, 10.1111/j.1438-8677.1985.tb01848.x
  17. Demesure B, Sodzi N, Petit RJ (1995) A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Molec Ecol 4:129
  18. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
  19. Ducarme V (2008) Ecologial and genetic aspects of natural hybridization in Rhinanthus L. PhD thesis, Louvain University, Louvain-la-Neuve
  20. Ducarme V, Wesselingh RA (2005) Detecting hybridization in mixed populations of Rhinanthus minor and Rhinanthus angustifolius. Folia Geobot 40:151–161
  21. Ducarme V, Wesselingh RA (2010) Performance of two Rhinanthus species under different hydric conditions. Pl Ecol 206:263–277
  22. Dumolin-Lapègue S, Pemonge M-H, Petit RJ (1997) An enlarged set of consensus primers for the study of organelle DNA in plants. Molec Ecol 6:393–397
  23. Erazzu LE, Camadro EL, Clausen AM (2009) Persistence over time, overlapping distribution and molecular indications of interspecific hybridization in wild potato populations of Northwest Argentina. Euphytica 168:249–262
  24. Ervin GN, Wetzel RG (2002) Influence of a dominant macrophyte, Juncus effusus, on wetland plant species richness, diversity, and community composition. Oecologia 130:626–636
  25. Fritz RS, Nichols-Orians CM, Brunsfeld SJ (1994) Interspecific hybridization of plants and resistance to herbivores – hypotheses, genetics, and variable responses in a diverse herbivore community. Oecologia 97:106–117
  26. Harrison RG (1990) Hybrid zones: windows on evolutionary process. In Futuyma D, Antonovics J (eds) Oxford Surveys in Evolutionary Biology 7. Oxford University Press, Oxford, pp 69–128
  27. Harrison RG (1993) Hybrids and hybrid zones: historical perspective. In Harrison RG (ed) Hybrid zones and the evolutionary process. Oxford University Press, New York, pp 3–12
  28. Huang SSF, Hwang SY, Lin TP (2002) Spatial pattern of chloroplast DNA variation of Cuclobalanopsis glauca in Taiwan and East Asia. Molec Ecol 11:2349–2358
  29. Johnston JA, Wesselingh RA, Bouck AC, Donovan LA, Arnold ML (2001) Intimately linked or hardly speaking? The relationship between genotype and environmental gradients in a Louisiana Iris hybrid population. Molec Ecol 10:673–681
  30. Kelly D (1989) Demography of short-lived plants in chalk grassland. 3. Population stability. J Ecol 77:785–798
  31. Knobloch IW (1972) Intergeneric hybridization in flowering plants. Taxon 21:97–103
  32. Kwak MM (1978) Pollination, hybridization and ethological isolation of Rhinanthus minor and R. serotinus (Rhinanthoideae: Scrophulariaceae) by bumblebees (Bombus Latr.). Taxon 27:145–158
  33. Kwak Manja M., EFFECTS OF BUMBLEBEE VISITS ON THE SEED SET OF PEDICULARIS, RHINANTHUS AND MELAMPYRUM (SCROPHULARIACEAE) IN THE NETHERLANDS, 10.1111/j.1438-8677.1979.tb00333.x
  34. Kwak MM (1979b) Maintenance of species integrity in sympatrically occurring Rhinanthus minor and Rhinanthus serotinus in the Netherlands. Oecologia 41:1–9
  35. Kwak MM (1980) Artificial and natural hybridization and introgression in Rhinanthus (Scrophulariaceae) in relation to bumblebee pollination. Taxon 29:613–628
  36. Kwak MM, Holthuijzen YA, Prins HHT (1985) A comparison of nectar characteristics of the bumblebee-pollinated Rhinanthus minor and Rhinanthus serotinus. Oikos 44:123–126
  37. Lambinon J, Delvosalle L, Duvignaud J (2004) Nouvelle flore de la Belgique, du Grand-Duché de Luxembourg, du Nord de la France et des régions voisines, Ed. 5 National Botanic Garden of Belgium, Meise
  38. Lepais O, Petit RJ, Guichoux E, Lavabre JE, Alberto F, Kremer A, Gerber S (2009) Species relative abundance and direction of introgression in oaks. Molec Ecol 18:2228–2242
  39. Minder AM, Rothenbuehler C, Widmer A (2007) Genetic structure of hybrid zones between Silene latifolia and Silene dioica (Caryophyllaceae): evidence for introgressive hybridization. Molec Ecol 16:2504–2516
  40. Nason JD, Ellstrand NC, Arnold ML (1992) Patterns of hybridization and introgression in populations of oaks, manzanitas, and irises. Amer J Bot 79:101–111
  41. Pellegrino G, Bellusci F, Musacchio A (2009) Genetic integrity of sympatric hybridising plant species: the case of Orchis italica and O. anthropophora. Pl Biol 11:434–441
  42. Perron M, Bousquet J (1997) Natural hybridization between black spruce and red spruce. Molec Ecol 6:725–734
  43. Perron M., Gordon A.G., Bousquet J., Species-specific RAPD fingerprints for the closely related Picea mariana and P. rubens, 10.1007/bf00220871
  44. Rameau JC, Mansion D, Dumé G (1989) Flore forestière française, guide écologique illustré. Vol. 1 Plaines et collines. Institut pour le Développement Forestier, Paris
  45. Repplinger M, Johannesen J, Seitz A, Comes HP (2007) Morphological and molecular evidence for hybridization and introgression in Central European Arctium (Asteraceae). Pl Syst Evol 268:75–95
  46. Rhymer JM, Simberloff D (1996) Extinction by hybridization and introgression. Annual Rev Ecol Syst 27:83–109
  47. Rieseberg LH (1991) Homoploid reticulate evolution in Helianthus (Asteraceae): evidence from ribosomal genes. Amer J Bot 78:1218–1237
  48. Rieseberg LH (1995) The role of hybridization in evolution – old wine in new skins. Amer J Bot 82:944–953
  49. Rieseberg Loren H., Brunsfeld Steven J., Molecular Evidence and Plant Introgression, Molecular Systematics of Plants (1992) ISBN:9780412022418 p.151-176, 10.1007/978-1-4615-3276-7_7
  50. Rieseberg LH, Wendel JF (1993) Introgression and its consequences in plants. In Harrison RG (ed) Hybrid zones and the evolutionary process. Oxford University Press, New York
  51. Sang T, Crawford DJ, Stuessy TF (1997) Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). Amer J Bot 84:1120–1136
  52. Siegel S, Castellan NJ, Jr (1988) Nonparametric statistics for the behavioral sciences, Ed. 2. McGraw-Hill, New York
  53. Sokal RR, Rohlf FJ (1995) Biometry, Ed. 3. Freeman, New York
  54. Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Pl Molec Biol 17:1105–1109
  55. van der Meijden R (1990) Heukels’ Flora van Nederland, Ed. 21. Wolters-Noordhoff, Groningen
  56. Van Landuyt W (1993) De Kalkense Meersen. Nieuwsbrief werkgroep Kalkense Meersen 1993:10–20
  57. Van Landuyt W, Hoste I, Vanhecke L, Van den Bremt P, Vercruysse W, De Beer D (2006) Atlas van de Flora van Vlaanderen en het Brussels Gewest. Instituut voor natuur- en bosonderzoek, Nationale Plantentuin van België & Flo.Wer, Brussel
  58. Vrancken J (2010) Individual and comparative phylogeography of Rhinanthus angustifolius and Rhinanthus minor in Europe. PhD thesis, Louvain University, Louvain-la-Neuve
  59. Vrancken J, Brochmann C, Wesselingh RA (2009) How did an annual plant react to Pleistocene glaciations? Postglacial history of Rhinanthus angustifolius in Europe. Biol J Linn Soc 98:1–13
  60. Watkinson AR, Gibson CC (1988) Plant parasitism: the population dynamics of parasitic plants and their effect upon plant community structure. In Davy AJ, Hutchings MJ, Watkinson AR (eds) Plant population ecology, The British Ecological Society. Blackwell, Oxford, pp 393–411
  61. Weeda EJ (1988) Ratelaar/Rhinanthus. In Weeda EJ, Westra R, Westra C, Westra T (eds) Nederlandse oecologische flora: wilde planten en hun relaties 3. IVN, Amsterdam, pp 234–237
  62. Westbury DB (2004) Biological Flora of the British Isles: Rhinanthus minor L. J Ecol 92:906–927
Bibliographic reference Ducarme, Véronique ; Vrancken, Jérôme ; Wesselingh, Renate A.. Hybridization in annual plants: patterns and dynamics during a four-year study in mixed Rhinanthus populations. In: Folia Geobotanica, Vol. 45, no. 4, p. 387-405 (2010)
Permanent URL http://hdl.handle.net/2078.1/75212