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A New Production Method for Glucose-oxidase

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Debaetselier, A. Dohet, P. De Beukelaer, Michèle [UCL] Hathi, V. Hanotier, J. Vasavada, A.

The recombinant yeast expression plasmid, pSGO2, has been constructed containing a hybrid yeast alcohol dehydrogenase II-glyceraldehyde-3-phosphate dehydrogenase promoter, the glucose oxidase presequence and the mature glucose oxidase coding sequence. When transformed into yeast, this plasmid directs the synthesis and secretion of more than 1.5 g l-1 of active glucose oxidase. Analysis of the yeast-derived enzyme shows extensive N-linked glycosylation in comparison to the A. niger protein. Both proteins show similar specific activities in terms of U mg-1 of protein.

Document type Communication à un colloque (Conference Paper) – Présentation orale avec comité de sélection
Access type Accès restreint
Publication date 1992
Language Anglais
Conference "INTERNATIONAL CONF ON THE INDUSTRIAL APPLICATIONS OF NATURAL, MODIFIED AND ARTIFICIAL ENZYMES", PISA(Italy) (Sep 23-29, 1990)
Journal information "Journal of Biotechnology" - Vol. 24, no. 2, p. 141-148 (1992)
Peer reviewed yes
issn 0168-1656
e-issn 1873-4863
Publisher Elsevier Science Bv (Amsterdam)
Affiliation UCL - MD/MNOP - Département de morphologie normale et pathologique
Keywords Saccharomyces-cerevisiae ; Recombinant Glucose Oxidase ; Secretion ; Purification
Links
Bibliographic reference Debaetselier, A. ; Dohet, P. ; De Beukelaer, Michèle ; Hathi, V. ; Hanotier, J. ; et. al. A New Production Method for Glucose-oxidase.INTERNATIONAL CONF ON THE INDUSTRIAL APPLICATIONS OF NATURAL, MODIFIED AND ARTIFICIAL ENZYMES (PISA(Italy), Sep 23-29, 1990). In: Journal of Biotechnology, Vol. 24, no. 2, p. 141-148 (1992)
Permanent URL http://hdl.handle.net/2078.1/63574