User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Adsorption of proteins on an AISI 316 stainless-steel surface in natural seawater

Primary tabs

Pradier, CM Bertrand, Patrick [UCL] Bellon-Fontaine, MN Compere, C Costa, D. Poleunis, Claude [UCL] Walls, MG

The surface of a metal immersed in solutions containing organic and inorganic molecules is modified by an adsorbed layer that influences the subsequent biofilm formation. Little is known about the first steps of surface modifications in the complex medium of seawater. The 'BASIS' group has characterized the conditioning film formed on a stainless-steel surface immersed in natural seawater. The chemical composition, morphology and growth mode of this film have been determined using complementary surface analytical techniques: x-ray photoelectron spectroscopy (XPS), infrared reflection absorption spectroscopy (IRAS), atomic force microscopy (AFM), surface free energy measurements and time-of-flight secondary ion mass spectrometry (ToF-SIMS), The XPS and ToF-SIMS data show that the primary film is mainly composed of proteins in the early stages of immersion. The SIMS, XPS, surface free energy and AFM data indicate that the adsorbed protein layer is discontinuous, leaving bare oxide areas. Our results and interpretations are supported by similar experiments in artificial seawater containing controlled amounts of proteins. Copyright (C) 2000 John Wiley & Sons, Ltd.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2000
Language Anglais
Journal information "Surface and Interface Analysis" - Vol. 30, no. 1, p. 45-49 (2000)
Peer reviewed yes
Publisher John Wiley & Sons Ltd (W Sussex)
issn 0142-2421
e-issn 1096-9918
Publication status Publié
Affiliation UCL - FSA/MAPR - Département des sciences des matériaux et des procédés
Keywords Biofilm ; Protein ; Adsorption ; Surfaces ; Xps ; Tof-sims
Links
  1. Mori Osamu, Imae Toyoko, AFM investigation of the adsorption process of bovine serum albumin on mica, 10.1016/s0927-7765(97)00005-2
  2. Schakenraad J.M., Busscher H.J., Cell—polymer interactions: The influence of protein adsorption, 10.1016/0166-6622(89)80349-x
  3. Elgersma Alfred V, Zsom Rob L.J, Norde Willem, Lyklema Johannes, The adsorption of bovine serum albumin on positively and negatively charged polystyrene latices, 10.1016/0021-9797(90)90190-y
  4. In Proc. 3rd Int. Cong. Marine Corrosion and Fouling, (ed). Northwestern University Press: Illinois, USA, 1973; 633.
  5. The Electrochemical Society Proceeding Series. PV 97-26. ECS: Pennington, NJ, 1998; 450.
  6. Bertrand, Mikrochim. Acta, 13, 167 (1996)
  7. Briggs D, Hearn MJ, Interaction of ion beams with polymers, with particular reference to SIMS, 10.1016/0042-207x(86)90156-9
  8. Taylor G T, Zheng D, Lee M, Troy P J, Gyananath G, Sharma S K, Influence of surface properties on accumulation of conditioning films and marine bacteria on substrata exposed to oligotrophic waters, 10.1080/08927019709378319
  9. Frølund B, Suci P A, Langille S, Weiner R M, Geesey G G, Influence of protein conditioning films on binding of a bacterial polysaccharide adhesin fromHyphomonasMHS‐3, 10.1080/08927019609386268
  10. Daeschel Mark A., McGuire Joseph, Interrelationships between Protein Surface Adsorption and Bacterial Adhesion, 10.1080/02648725.1998.10647964
Bibliographic reference Pradier, CM ; Bertrand, Patrick ; Bellon-Fontaine, MN ; Compere, C ; Costa, D. ; et. al. Adsorption of proteins on an AISI 316 stainless-steel surface in natural seawater. In: Surface and Interface Analysis, Vol. 30, no. 1, p. 45-49 (2000)
Permanent URL http://hdl.handle.net/2078.1/62109