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Expression of galectin-3 in primary and metastatic melanoma: immunohistochemical studies on human lesions and nude mice xenograft tumors

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Vereecken, P Debray, C Petein, M. Awada, A. Lalmand, MC Heenen, M

Galectins are a large family of proteins which bind galactoside-containing glycans. Their role in cancer seems to be important since members of the family may mediate cell adhesion and modulate cell growth. Galectin-3 (Gal-3) is expressed in the nucleus, in the cytoplasm and on the cell surface, and can also be secreted into the extracellular matrix. A series of experimental and clinical data have been reported which indicate that Gal-3 may play a putative role in carcinogenesis, cancer progression and the process of metastasis. To study the possible correlation between Gal-3 expression and malignant potential in primary melanoma lesions, we conducted an immunohistochemical study with monoclonal anti-Gal-3 antibody in a series of primary and metastatic melanoma lesions as well as benign skin pigmented lesions. We also developed a xenograft melanoma model in nude mice with two melanoma cell lines (ATCC G-361 and ATCC HT-144) and assessed staining with the Gal-3 antibody in the xenografts and the metastases. The expression of anti-Gal-3 staining was determined semiquantitatively. The expression of Gal-3 was higher in thin primary melanoma lesions than in benign pigmented skin lesions or metastases and seemed to correlate inversely with the aggressiveness as estimated by the Breslow index which is recognized as the main prognostic factor in melanoma. We propose Gal-3 expression in melanoma as a diagnostic and/or a prognostic parameter and suggest that further studies of such a role for Gal-3 are warranted.

Document type Communication à un colloque (Conference Paper) – Présentation orale avec comité de sélection
Access type Accès restreint
Publication date 2005
Language Anglais
Conference "Fall Meeting of the Melanoma-Group of the European-Organization-for-Research-and-Treatment-of-Cancer", Berlin(Germany) (Sep 24, 2004)
Journal information "Archives of Dermatological Research" - Vol. 296, no. 8, p. 353-358 (2005)
Peer reviewed yes
issn 0340-3696
e-issn 1432-069X
Publisher Springer (New York)
Affiliation UCL - Autre
Keywords Melanoma ; Galectin-3 ; Galactose-binding Protein
Links
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Bibliographic reference Vereecken, P ; Debray, C ; Petein, M. ; Awada, A. ; Lalmand, MC ; et. al. Expression of galectin-3 in primary and metastatic melanoma: immunohistochemical studies on human lesions and nude mice xenograft tumors.Fall Meeting of the Melanoma-Group of the European-Organization-for-Research-and-Treatment-of-Cancer (Berlin(Germany), Sep 24, 2004). In: Archives of Dermatological Research, Vol. 296, no. 8, p. 353-358 (2005)
Permanent URL http://hdl.handle.net/2078.1/61134