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Interaction of the Yeast Pleiotropic Drug-resistance Gene-pdr1 and Gene-pdr5

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Document type : Article de périodique (Journal article) – Article de recherche
Abstract :

The network of genes which mediates multiple drug resistance in yeast includes, among others, the PDR1 gene, which encodes a putative regulator of gene expression, and PDR5, a locus whose amplification leads to resistance. We demonstrate that disruption of PDR5 causes marked hypersensitivity not only to cycloheximide but also to sulphometuron methyl and the mitochondrial inhibitors chloramphenicol, lincomycin, erythromycin and antimycin. Genetic analysis of double mutants containing an insertion in PDR5 (pdr5:Tn5), which renders cells hypersensitive to cycloheximide, and a pdr1 mutation, which confers resistance to this inhibitor, indicates that the expression of resistance requires a functional PDR5 gene. The same interdependency is observed for chloramphenicol, but not for oligomycin, lincomycin. erythromycin or sulphometuron methyl. Northern analysis of PDR1 and PDR5 transcripts reveals that the 5.2 kbp PDR5 transcript is overexpressed in pdr1 (resistant) mutants, but underexpressed in a disruption of PDR1. These observations provide strong experimental support for our former proposal that the PDR5 gene is a target for regulation by the PDR1 gene product.
Access type : Accès restreint
Publication date : 1992
Language : Anglais
Journal information : "Current Genetics : lower eukaryotes and organelles" - Vol. 21, no. 6, p. 431-436 (1992)
Peer reviewed : yes
Publisher : Springer Verlag (New York)
issn : 0172-8083
e-issn : 1432-0983
Publication status : Publié
Affiliation : UCL
Keywords : DRUG RESISTANCE ; YEAST ; POSITIVE ACTIVATOR
Links :
Bibliographic reference Meyers, S. ; Schauer, W. ; Balzi, E. ; Wagner, Marisa ; Goffeau, André ; et. al. Interaction of the Yeast Pleiotropic Drug-resistance Gene-pdr1 and Gene-pdr5. In: Current Genetics : lower eukaryotes and organelles, Vol. 21, no. 6, p. 431-436 (1992)
Permanent URL http://hdl.handle.net/2078.1/50407

References Provided by I4OC

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