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Mouse-tumor rejection antigen-p815a and antigen-p815b: two epitopes carried by a single peptide

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Lethe, Bernard [UCL] Van den Eynde, Benoît [UCL] Van Pel, Aline [UCL] Corradin, Giampietro [LICR Lausanne] Boon, Thierry [UCL]

Mouse mastocytoma P815 expresses several distinct tumor rejection antigens recognized by syngeneic cytolytic T lymphocytes (CTL). Two of these tumor rejection antigens, P815A and P815B, are encoded by gene PIA, the sequence of which was reported previously. Tumor cell variants having lost one or both of these antigens were isolated by in vitro selection with CTL and also by collecting tumor cells that progressed in vivo after escaping a nearly complete immune rejection process. The structure of gene PIA in these antigen-loss variants was examined. Several A-B- variants presented a complete or partial deletion of the gene. One variant that had lost only antigen A displayed a point mutation in the first exon. Peptides were synthesized that corresponded to the normal sequence located in the region of this point mutation. They sensitized target cells to both anti-A and anti-B CTL.The homologous peptide encoded by the mutated gene of the P815 A-B+ variant sensitized cells only to anti-B CTL. We conclude that anti-A and anti-B CTL recognize on the same peptide two distinct epitopes that are affected differently by the mutation.

Document type Article de périodique (Journal article)
Publication date 1992
Language Anglais
Journal information "European Journal of Immunology" - Vol. 22, no. 9, p. 2283-2288 (1992)
Peer reviewed yes
Publisher Vch Publishers Inc (Deerfield Beach)
issn 0014-2980
e-issn 1521-4141
Publication status Publié
Affiliation UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
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Bibliographic reference Lethe, Bernard ; Van den Eynde, Benoît ; Van Pel, Aline ; Corradin, Giampietro ; Boon, Thierry. Mouse-tumor rejection antigen-p815a and antigen-p815b: two epitopes carried by a single peptide. In: European Journal of Immunology, Vol. 22, no. 9, p. 2283-2288 (1992)
Permanent URL http://hdl.handle.net/2078.1/50241