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Cloning and Chromosomal Localization of a Pseudogene Corresponding To a Messenger-rna for a Soluble Il-6 Receptor

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Snyers, L. Renauld, Jean-Christophe [UCL] Szpirer, C. Content, J.

A polymerase chain reaction assay (Lust J.A. et al. (1992). Cytokine 4:96-100) was used to detect a mRNA coding for a soluble IL-6 receptor in human hepatoma cells. In addition to the expected amplification product, we found a sequence (SR4) which could be aligned to it with 78% identity. After cloning and sequencing a genomic 2.5-2.7 kB EcoRI fragment containing SR4, this sequence turned out to be part of a pseudogene corresponding to the transmembrane domain deleted soluble IL-6 receptor. Screening of a panel of interspecies hybrids revealed that it maps to chromosome 9.

Document type Article de périodique (Journal article)
Publication date 1995
Language Anglais
Journal information "DNA Sequence" - Vol. 5, no. 5, p. 311-314 (1995)
Peer reviewed yes
Publisher Harwood Acad Publ Gmbh (Reading)
issn 1042-5179
e-issn 1029-2365
Publication status Publié
Affiliation UCL
Keywords SOLUBLE IL-6 RECEPTOR ; PSEUDOGENE
Links
Bibliographic reference Snyers, L. ; Renauld, Jean-Christophe ; Szpirer, C. ; Content, J.. Cloning and Chromosomal Localization of a Pseudogene Corresponding To a Messenger-rna for a Soluble Il-6 Receptor. In: DNA Sequence, Vol. 5, no. 5, p. 311-314 (1995)
Permanent URL http://hdl.handle.net/2078.1/47977