Farina, C.
van der Bruggen, Pierre
[UCL]
Boël, P.
Parmiani, G.
Sensi, M.
In this study we determined TCR alpha and beta chain nucleotide sequences of HLA-Cw*1601-restricted cytotoxic T lymphocyte (CTL) clones obtained from the peripheral blood lymphocytes (PBL) of a melanoma patient. These clones were previously shown to be involved in the recognition of melanoma-associated antigenic epitopes SAYGEPRKL and AARAVFLAL encoded by gene MAGE-1 and BAGE respectively. All (3/3) anti-MAGE-1 CTL clones displayed TCRBV5 usage and one clonotype was found twice, >1 year apart, in patient's PBL. Two out of three anti-BAGE CTL clones showed the same TCRAV/AJ and TCRBV/BJ combinations and differed in the alpha chain CDR3 for two residues and in the beta chain CDR3 for a single nucleotide which, however, did not change translation, These results suggest a pattern of TCR conservation in CTL selected for recognition of MAGE-1 or BAGE peptides on the autologous melanoma.
Bibliographic reference |
Farina, C. ; van der Bruggen, Pierre ; Boël, P. ; Parmiani, G. ; Sensi, M.. Conserved TCR usage by HLA-Cw*1601-restricted T cell clones recognizing melanoma antigens. In: International Immunology, Vol. 8, no. 9, p. 1463-1466 (1996) |
Permanent URL |
http://hdl.handle.net/2078.1/46910 |