User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Functional analysis of three adjacent open reading frames from the right arm of yeast: Chromosome XVI

Primary tabs

Waskiewicz-Staniorowska, B Skala, J. Jasinski, M Grenson, M. Goffeau, André [UCL] Ulaszewski, S.

A 7.24 kb genomic DNA fragment from the yeast Saccharomyces cerevisiae chromosome XVI was isolated by complementation of a new temperature-sensitive mutation tsa1. We determined the nucleotide sequence of this fragment located on the right arm of chromosome XVI. Among the three, complete open reading frames: YPR041w, YPR042c and YPR043w contained within this fragment, the gene YPR041w was shown to complement the tsa1 mutation and to correspond to the TIF5 gene encoding an essential protein synthesis initiation translation factor. The YPR042c gene encodes a hypothetical protein of 1075 amino acids containing four putative transmembrane segments and is non-essential for growth. The gene YPR043c encoding the 10 kDa product, highly similar to the human protein L37a from the 60S ribosomal subunit, was found to be essential and a dominant lethal. We conclude that three tightly linked yeast genes are involved in the translation process. (C) 1998 John Wiley & Sons, Ltd.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 1998
Language Anglais
Journal information "Yeast : a forum for yeast researchers" - Vol. 14, no. 11, p. 1027-1039 (1998)
Peer reviewed yes
Publisher John Wiley & Sons Ltd (W Sussex)
issn 0749-503X
e-issn 1097-0061
Affiliation UCL
Keywords Saccharomyces cerevisiae ; gene cloning ; gene disruption ; functional analysis ; chromosome XVI ; translation
Links
  1. Balzi, J. Biol. Chem., 262, 16871 (1987)
  2. Balzi, Biochim. Biophys. Acta, 1187, 152 (1994)
  3. Baronas-Lowell D M, Warner J R, Ribosomal protein L30 is dispensable in the yeast Saccharomyces cerevisiae., 10.1128/mcb.10.10.5235
  4. Berg, Science, 232, 485 (1986)
  5. Bolivar, Gene, 2, 95 (1977)
  6. Bonneaud, Yeast, 7, 609 (1991)
  7. Capieaux, J. Biol. Chem., 264, 7437 (1989)
  8. Chakravarti, J. Biol. Chem., 268, 10524 (1993)
  9. Chang, J. Cell Biol., 115, 289 (1991)
  10. Culbertson, Genetics, 80, 23 (1975)
  11. Delhez, Eur. J. Biochem., 79, 319 (1977)
  12. Fickett, Nucl. Acids Res., 10, 5303 (1982)
  13. Foury, J. Biol. Chem., 250, 2354 (1975)
  14. Gerbaud, Curr. Genet., 3, 173 (1981)
  15. Goffeau André, Slayman Carolyn W., The proton-translocating ATPase of the fungal plasma membrane, 10.1016/0304-4173(81)90010-0
  16. Hanahan, J. Mol. Biol., 116, 557 (1983)
  17. Harris, Proc. Natl. Acad. Sci. USA, 91, 10531 (1994)
  18. Ito, J. Bacteriol., 153, 163 (1983)
  19. and (Eds) (1994). Methods in Yeast Genetics. Cold Spring Harbor Laboratories, Cold Spring Harbor, New York.
  20. Klein, Biochem. Biophys. Acta, 815, 468 (1985)
  21. Lowry, J. Biol. Chem., 193, 265 (1951)
  22. Nakai, Genomics, 14, 897 (1992)
  23. McCusker J H, Perlin D S, Haber J E, Pleiotropic plasma membrane ATPase mutations of Saccharomyces cerevisiae., 10.1128/mcb.7.11.4082
  24. Mortimer, Yeast, 5, 321 (1989)
  25. Pearson, Proc. Natl. Acad. Sci. USA, 85, 2444 (1988)
  26. Pikielny, Cell, 34, 396 (1983)
  27. Mohana Rao J.K., Argos Patrick, A conformational preference parameter to predict helices in integral membrane proteins, 10.1016/0167-4838(86)90295-5
  28. Rao, Yeast, 9, 1075 (1993)
  29. Rogers, Science, 234, 364 (1986)
  30. Roon, Arch. Biochem. Biophys., 185, 142 (1978)
  31. Rothstein, Meth. Enzymol., 101, 202 (1983)
  32. and (1992). Yeast premRNA splicing. In and (Eds), The Molecular and Cellular Biology of the Yeast Saccharomyces cerevisiae, vol. 2. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp. 4/143–4/192.
  33. and (Eds) (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratories, Cold Spring Harbor, New York.
  34. Sanger, Proc. Natl. Acad. Sci. USA., 74, 5463 (1977)
  35. Schwarz, J. Mol. Biol., 179, 125 (1984)
  36. Serrano, FEBS Lett., 156, 11 (1983)
  37. Serrano, Nature, 319, 493 (1986)
  38. Serrano, Annu. Rev. Plant Physiol. Plant Molec. Biol., 40, 61 (1989)
  39. Sharp, Nucl. Acids Res., 15, 1281 (1987)
  40. Song, Curr. Gen., 30, 273 (1996)
  41. Ułaszewski, Eur. J. Biochem., 130, 235 (1983)
  42. Ułaszewski, Molec. Gen. Genet., 207, 38 (1987a)
  43. Ułaszewski, J. Biol. Chem., 262, 223 (1987b)
  44. Vallejo, Yeast, 5, 307 (1989)
  45. Wach, J. Bioenerg. Biomembr., 24, 309 (1992)
  46. Woolford, Yeast, 5, 439 (1989)
  47. and (1991). The ribosome and its synthesis. In and (Eds), The Molecular and Cellular Biology of the Yeast Saccharomyces cerevisiae, vol. 1. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp. 10/587–10/626.
Bibliographic reference Waskiewicz-Staniorowska, B ; Skala, J. ; Jasinski, M ; Grenson, M. ; Goffeau, André ; et. al. Functional analysis of three adjacent open reading frames from the right arm of yeast: Chromosome XVI. In: Yeast : a forum for yeast researchers, Vol. 14, no. 11, p. 1027-1039 (1998)
Permanent URL http://hdl.handle.net/2078.1/45128