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Survival and metabolic function of freshly isolated rat hepatocytes exposed first to a heat shock and then to an oxidative stress

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Latour, I Buc Calderon, Pedro [UCL]

The formation of heat shock proteins (hsp) leading to thermotolerance has been extensively reported in many cell types. In freshly isolated rat hepatocytes, hsp were synthesized after 60 minutes of incubation at 42 degrees C. Cell survival was not modified by such a treatment, but protein synthesis, secretion of triglycerides as lipoproteins, and the maintenance of both ATP and glycogen levels were significantly impaired. When exposed to an oxidative stress, heat-shocked hepatocytes were not more resistant than cells always kept at 37 degrees C. Conversely, the addition of tert-butyl hydroperoxide (tBOOH) resulted, in general, in an increased lactate dehydrogenase leakage. The metabolism of tBOOH, as estimated by the reduced glutathione (GSH) content and GSH peroxidase activity, was similar in both control and heat-shocked hepatocytes. Despite the synthesis of hsp in rat hepatocytes, the lack of resistance to a subsequent oxidant injury may be due to the metabolic impairment caused by the heat shock.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 1999
Language Anglais
Journal information "International Journal of Toxicology" - Vol. 18, no. 4, p. 239-244 (1999)
Peer reviewed yes
Publisher Taylor & Francis Ltd (London)
issn 1091-5818
e-issn 1092-874X
Publication status Publié
Affiliation UCL - MD/FARM - Ecole de pharmacie
Keywords heat shock proteins ; oxidative stress ; rat hepatocytes ; cytotoxicity ; hydroperoxides ; liver metabolism
Links
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Bibliographic reference Latour, I ; Buc Calderon, Pedro. Survival and metabolic function of freshly isolated rat hepatocytes exposed first to a heat shock and then to an oxidative stress. In: International Journal of Toxicology, Vol. 18, no. 4, p. 239-244 (1999)
Permanent URL http://hdl.handle.net/2078.1/44191