Acetate utilization in Lactococcus lactis deficient in lactate dehydrogenase: a rescue pathway for maintaining redox balance

Hols, Pascal [UCL] Ramos, A Hugenholtz, J Delcour, Jean [UCL] de Vos, WM Santos, H Kleerebezem, M et al.[show all]

Acetate was shown to improve glucose fermentation in Lactococcus lactis deficient in lactate dehydrogenase. C-13 and H-1 nuclear magnetic resonance studies using [2-C-13]glucose and [2-C-13]acetate as substrates demonstrated that acetate was exclusively converted to ethanol. This novel pathway provides an alternative route for NAD(+) regeneration in the absence of lactate dehydrogenase.