Tsuboi, Kazuhito
[Kagawa University School of Medicine]
Hilligsmann, Christine
[UCL]
Vandevoorde, Severine
[UCL]
Lambert, Didier
[UCL]
Ueda, Natsuo
[Kagawa University School of Medicine]
Anandamide (N-arachidonoylethanolamine) and other bioactive N-acylethanolamines are degraded to their corresponding fatty acids and ethanolamine. This hydrolysis is mostly attributed to catalysis by FAAH (fatty acid amide hydrolase), which exhibits an alkaline pH optimum. In addition, we have identified another amidase which catalyses the same reaction exclusively at acidic pH values [Ueda. Yamanaka and Yamamoto (2001) J. Biol. Chem. 276, 35552-35557]. In attempts to find selective inhibitors of this acid amidase, we screened various derivatives of palmitic acid, 1-hexadecanol. and 1-pentadecylamine with N-palmitoylethanolamine as substrate. Here we show that N-cyclohexanecarbonyl-pentadecylamine inhibits the acid amidase from rat lung with an IC50 of 4.5 muM, without inhibiting FAAH at concentrations Lip to 100 muM. The inhibition was reversible and non-competitive. This compound also inhibited the acid amidase in intact alveolar macrophages. With the aid of this inhibitor, it was revealed that rat basophilic leukaemia cells possess the acid amidase as well as FAAH. Thus the inhibitor may be a useful tool to distinguish the acid amidase from FAAH in various tissues and cells and to elucidate the physiological role of the enzyme.
Bibliographic reference |
Tsuboi, Kazuhito ; Hilligsmann, Christine ; Vandevoorde, Severine ; Lambert, Didier ; Ueda, Natsuo. N-cyclohexanecarbonylpentadecylamine: a selective inhibitor of the acid amidase hydrolysing N-acylethanolamines, as a tool to distinguish acid amidase from fatty acid amide hydrolase. In: Biochemical Journal, Vol. 379, p. 99-106 (2004) |
Permanent URL |
http://hdl.handle.net/2078.1/40248 |