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Lupus anticoagulant testing using plasma spiked with monoclonal antibodies: performance in the UKNEQAS proficiency testing programme

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Jennings, I Mackie, I Arnout, Jef [UCL] Preston, FE

We report here results from a United Kingdom National Quality Assessment Scheme (UK NEQAS) exercise in which both plasma spiked with monoclonal antibodies and plasma from a patient known to have lupus anticoagulant (LA) were distributed to 245 hemostasis laboratories with a request for them to test for possible LA using their routine screening procedure. In general, good agreement was seen in the diagnosis of samples spiked with monoclonal antibodies against beta2-glycoprotein 1 (beta2GP1) and prothrombin, the LA-positive patient sample, and a normal pooled plasma; over 87% of centers correctly identified each sample. However, methods employing platelet neutralizing procedures were associated with a higher proportion of false-negative responses with the antiprothrombin-spiked sample, and it is important to recognize that sensitivity and responsiveness of different methods may vary between artificial plasmas and different LA-positive patient plasmas.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2004
Language Anglais
Journal information "Journal of Thrombosis and Haemostasis" - Vol. 2, no. 12, p. 2178-2184 (2004)
Peer reviewed yes
Publisher Blackwell Publ Ltd (Oxford)
issn 1538-7933
e-issn 1538-7836
Publication status Publié
Affiliation UCL
Keywords lupus anticoagulant ; monoclonal antibodies ; quality control
Links
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Bibliographic reference Jennings, I ; Mackie, I ; Arnout, Jef ; Preston, FE. Lupus anticoagulant testing using plasma spiked with monoclonal antibodies: performance in the UKNEQAS proficiency testing programme. In: Journal of Thrombosis and Haemostasis, Vol. 2, no. 12, p. 2178-2184 (2004)
Permanent URL http://hdl.handle.net/2078.1/39649