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Selective identification of HLA-DP4 binding T cell epitopes encoded by the MAGE-A gene family

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Wang, Xiao-Fei Cohen, William M. Castelli, Florence A. Almunia, Christine Lethe, Bernard van der Bruggen, Pierre [UCL] Maillere, Bernard

Because of the high frequency of HLA-DP4 in the Caucasian population, we have selectively delineated HLA-DP4 restricted T cell epitopes in the MAGE-A tumor antigens. We identified 12 good binders to HLA-DP4 and investigated the capacity of the seven best binders to induce in vitro specific CD4+ T cell lines from HLA-DP4 healthy donors. We found that the MAGE-A1 90-104 peptide exhibited a high and constant frequency of CD4+ T cell precursors in all the six tested donors. The MAGE-A1 268-282 peptide was found immunogenic in only two donors but with a high precursor frequency. The MAGE-A12 127-141 peptide was T cell stimulating in six different donors and induced fewer T cell lines. The peptide-specific T cell lines were stimulated by DC loaded with the lysates of cells transfected with MAGE-A1 or MAGE-A12, or loaded with the recombinant protein. We also show that the immunoreactivity of CD4+ T cell epitopes restricted to the same HLA II molecule may vary from one individual to another, as a result of inter-individual variations in the CD4+ T cell repertoire.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2007
Language Anglais
Journal information "Cancer Immunology Immunotherapy" - Vol. 56, no. 6, p. 807-818 (2007)
Peer reviewed yes
Publisher Springer (New York)
issn 0340-7004
e-issn 1432-0851
Publication status Publié
Affiliation UCL - Autre
Keywords HLA-DP4 ; antigens/peptides/epitopes ; CD4+T lymphocyte ; tumor ; MAGE
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Bibliographic reference Wang, Xiao-Fei ; Cohen, William M. ; Castelli, Florence A. ; Almunia, Christine ; Lethe, Bernard ; et. al. Selective identification of HLA-DP4 binding T cell epitopes encoded by the MAGE-A gene family. In: Cancer Immunology Immunotherapy, Vol. 56, no. 6, p. 807-818 (2007)
Permanent URL http://hdl.handle.net/2078.1/37695