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Effects of aspergillus niger inoculum concentration upon the kinetics of starchy wastewater pretreatment in a tanks-in-series bioreactor under transitory conditions

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Coulibaly, L. Agathos, Spiros N. [UCL]

This paper discusses the effects of three Aspergillus niger inoculum concentrations (0.12, 2.3 and 3.6 g/l) upon the kinetics of starch pretreatment under aerobic and transitory conditions using a tanks-in-series reactor. A synthetic wastewater containing starch as model polysaccharide was fed into the reactor system to study this Polymer transformation by Aspergillus niger. Starch and metabolites (oligosaccharides with a molecular weight lower than 1 kDa) in the individual reactors were quantified respectively by the starch iodine complex (SIC) and anthrone methods. Enzyme activities were characterised with APT ZYM kits. Starch degradation and metabolite accumulation were both influenced by both fungal inoculum and reactor HRT. Starch degradation improved from 34 to 99% with a parallel in increase inoculum concentration from 0.12 to 3.6 g/l. An overall of 400 mg/l of metabolites accumulated in the reactor system. A. niger secreted both extracellular and cell-wall-bound enzymes among which amylases.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2007
Language Anglais
Journal information "Brazilian Journal of Chemical Engineering" - Vol. 24, no. 4, p. 499-507 (2007)
Peer reviewed yes
Publisher Brazilian Soc Chemical Eng (Sao Paulo)
issn 0104-6632
e-issn 1678-4383
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
Keywords starch ; pretreatment ; bioreactor ; enzyme ; aspergillus niger
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Bibliographic reference Coulibaly, L. ; Agathos, Spiros N.. Effects of aspergillus niger inoculum concentration upon the kinetics of starchy wastewater pretreatment in a tanks-in-series bioreactor under transitory conditions. In: Brazilian Journal of Chemical Engineering, Vol. 24, no. 4, p. 499-507 (2007)
Permanent URL http://hdl.handle.net/2078.1/37216