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Crystal structure of the IL-22/IL-22R1 complex and its implications for the IL-22 signaling mechanism

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Bleicher, Lucas de Moura, Patricia Ribeiro Watanabe, Leandra Colau, Didier Dumoutier, Laure [UCL] Renauld, Jean-Christophe [UCL] Polikarpov, Igor

Interleukin-22 (IL-22) is a member of the interleukin-10 cytokine family, which is involved in anti-microbial defenses, tissue damage protection and repair, and acute phase responses. Its signaling mechanism involves the sequential binding of IL-22 to interleukin-22 receptor 1 (IL-22R1), and of this dimer to interleukin-10 receptor 2 (IL-10R2) extracellular domain. We report a 1.9 A crystal structure of the IL-22/IL-22R1 complex, revealing crucial interacting residues at the IL-22/IL-22R1 interface. Functional importance of key residues was confirmed by site-directed mutagenesis and functional studies. Based on the X-ray structure of the binary complex, we discuss a molecular basis of the IL-22/IL-22R1 recognition by IL-10R2.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2008
Language Anglais
Journal information "FEBS Letters" - Vol. 582, no. 20, p. 2985-2992 (2008)
Peer reviewed yes
Publisher Elsevier Science Bv (Amsterdam)
issn 0014-5793
e-issn 1873-3468
Publication status Publié
Affiliation UCL
Keywords cytokines ; IL-22R1 ; IL-22 ; interleukins ; immunology ; X-ray crystallography
Links
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Bibliographic reference Bleicher, Lucas ; de Moura, Patricia Ribeiro ; Watanabe, Leandra ; Colau, Didier ; Dumoutier, Laure ; et. al. Crystal structure of the IL-22/IL-22R1 complex and its implications for the IL-22 signaling mechanism. In: FEBS Letters, Vol. 582, no. 20, p. 2985-2992 (2008)
Permanent URL http://hdl.handle.net/2078.1/36384