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beta-Carotene is incorporated or mobilized along with triglycerides in bovine adipose tissue in response to insulin or epinephrine

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Arias, E. Gonzalez, A. Shimada, A. Varela-Echavarria, A. Ruiz-Lopez, F. During, Alexandrine [UCL] Mora, O.

Pasture fed cattle ingest substantial amounts of beta-carotene (beta-C). Not all of the carotenoid compound is transformed into vitamin A, but the surplus is deposited in adipose tissue (AT). The mechanisms of beta-C incorporation and mobilization are unknown. Two experiments were conducted using explants from bovine AT cultured in vitro. First, beta-C incorporation by explants from three animals was examined with different beta-C concentrations (0, 1, 5 and 20 mu m) and different times of incubation (every 5 h up to 25 h). The data showed a significant increase of beta-C concentration in explants only for 20 mu m beta-C. Secondly, effects of insulin and epinephrine on beta-C and triglyceride (TG) contents of explants were studied. Explants from six animals were incubated with either hormone and 0 or 20 mu m beta-C for 20 h. Both TG and beta-C contents were affected positively by insulin and negatively by epinephrine. Interestingly, changes in ratios of beta-C/TG (hormone vs. control) were similar (1.7 x 10(-3) and 1.8 x 10(-3)), respectively, for insulin and epinephrine, indicating that beta-C level is directly related to TG content. We also report the presence of mRNA for beta-C 15, 15' oxygenase in bovine AT. The in vitro culture system using explants from bovine AT is a promising model to investigate factors that might affect the accumulation and metabolism of beta-C.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2009
Language Anglais
Journal information "Journal of Animal Physiology and Animal Nutrition" - Vol. 93, no. 1, p. 83-93 (2009)
Peer reviewed yes
Publisher Blackwell Publishing (Oxford)
issn 0931-2439
e-issn 1439-0396
Publication status Publié
Affiliation UCL
Keywords Bovine ; kidney adipose tissue explant ; beta-carotene ; triglycerides ; epinephrine ; insulin
Links
  1. Barrón, Técnica Pecuaria en México, 44, 231 (2006)
  2. Barua, Methods Enzymology, 213, 273 (1992)
  3. Bidner T. D., Schupp A. R, Mohamad A. B., Rumore N. C., Montgomery R. E., Bagley C. P., McMillin K. W., Acceptability of Beef from Angus-Hereford or Angus-Hereford-Brahman Steers Finished on All-Forage or a High-Energy Diet, 10.2527/jas1986.622381x
  4. Boone C, Grégoire F, Remacle C, Culture of porcine stromal-vascular cells in serum-free medium: differential action of various hormonal agents on adipose conversion., 10.2527/2000.784885x
  5. Bradford, Annals Biochemistry, 72, 248 (1976)
  6. Castro-Muñozledo, Differentiation, 36, 211 (1987)
  7. Di Francesco, Biochemical Pharmacology, 34, 3683 (1985)
  8. During, Annals Biochemistry, 241, 199 (1996)
  9. During, Biochemical and Biophysical Research Communications, 249, 467 (1998)
  10. During, Journal of Lipid Research, 43, 1086 (2002)
  11. During, The Journal of Nutrition, 135, 2305 (2005)
  12. Estado de Queretaro., Ley Estatal de Protección a los Animales (1996)
  13. Etherton Terry D., Evock Christina M., Stimulation of Lipogenesis in Bovine Adipose Tissue by Insulin and Insulin-Like Growth Factor, 10.2527/jas1986.622357x
  14. Forrest, Canadian Journal of Animal Science, 61, 575 (1981)
  15. Holm, Annual Review of Nutrition, 20, 365 (2000)
  16. KAWADA Teruo, TAKAHASHI Nobuyuki, FUSHIKI Tohru, Biochemical and Physiological Characteristics of Fat Cell., 10.3177/jnsv.47.1
  17. Kiefer, Proceedings of the National Academy of Science, 99, 10581 (2002)
  18. Knight, Proceedings of New Zealand Society of Animal Production, 53, 455 (1993)
  19. Kuri-Harcuch, Revista de Investigación Clínica (Mexico), 36, 377 (1984)
  20. Lalikos, The Journal of Surgical Research, 70, 95 (1997)
  21. Lancrajan, Chemistry and Physics of Lipids., 112, 1 (2001)
  22. McCaughey, Canadian Journal of Animal Science, 76, 149 (1996)
  23. McNamara, Journal of Dairy Science, 71, 643 (1988)
  24. McNamara, Journal of Dairy Science, 72, 407 (1989)
  25. McNamara, Journal of Dairy Science, 69, 3042 (1986)
  26. Mora, International Journal of Vitamin and Nutrition Research, 70, 199 (2000)
  27. Mora O., Romano J. L., González E., Ruiz F. J., Gómez R., Shimada A., Presence of fed β-carotene in digesta, excreta, blood, and hepatic and adipose tissues of Holstein steers, 10.4141/a00-004
  28. Mora-Izaguirre, International Journal of Vitamin and Nutrition Research, 74, 116 (2004)
  29. Moustaid, The Journal of Nutrition, 126, 865 (1996)
  30. Paik, Journal of Biological Chemistry, 276, 32160 (2001)
  31. Plaas, Journal of Developmental Physiology, 2, 275 (1980)
  32. Pu, Annals of Plastic Surgery, 54, 288 (2005)
  33. Pu, Plastic and Reconstructive Surgery, 117, 1845 (2006)
  34. Reboul, Biochemical Journal, 387, 455 (2005)
  35. Rosas, Expression of 15, 15′dioxigenase in bovine ovarian tissue. (2003)
  36. SAS OnlineDoc® 9.1.3 (2004)
  37. Simonne, Journal of Food Science, 61, 1254 (1996)
  38. Strachan, Australian Journal of Experimental Agriculture, 33, 269 (1993)
  39. Tondu, The Journal of Biological Chemistry, 280, 33536 (2005)
  40. Turinsky, The Journal of Biological Chemistry, 265, 16880 (1990)
  41. Van Bennekum, Biochemistry, 44, 4517 (2005)
  42. Wise, The Journal of Biological Chemistry, 254, 273 (1979)
  43. Wu, Journal of Veterinary Medical Science, 62, 933 (2000)
  44. Wyss, Biochemical Journal, 354, 521 (2001)
  45. Yang, Australian Journal of Agricultural Research, 44, 215 (1993)
Bibliographic reference Arias, E. ; Gonzalez, A. ; Shimada, A. ; Varela-Echavarria, A. ; Ruiz-Lopez, F. ; et. al. beta-Carotene is incorporated or mobilized along with triglycerides in bovine adipose tissue in response to insulin or epinephrine. In: Journal of Animal Physiology and Animal Nutrition, Vol. 93, no. 1, p. 83-93 (2009)
Permanent URL http://hdl.handle.net/2078.1/35888