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High Expression of Thyroid Hormone Receptors and Mitochondrial Glycerol-3-phosphate Dehydrogenase in the Liver Is Linked to Enhanced Fatty Acid Oxidation in Lou/C, a Rat Strain Resistant to Obesity

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Taleux, Nellie Guigas, Bruno [UCL] Dubouchaud, Herve Moreno, Maria Weitzel, Joachim M. Leverve, Xavier M.

Besides its well recognized role in lipid and carbohydrate metabolisms, glycerol is involved in the regulation of cellular energy homeostasis via glycerol-3-phosphate, a key metabolite in the translocation of reducing power across the mitochondrial inner membrane with mitochondrial glycerol-3-phosphate dehydrogenase. Here, we report a high rate of gluconeogenesis from glycerol and fatty acid oxidation in hepatocytes from Lou/C, a peculiar rat strain derived from Wistar, which is resistant to age- and diet-related obesity. This feature, associated with elevated cellular respiration and cytosolic ATP/ADP and NAD(+)/NADH ratios, was linked to a high expression and activity of mitochondrial glycerol-3-phosphate dehydrogenase. Interestingly, this strain exhibited high expression and protein content of thyroid hormone receptor, whereas circulating thyroid hormone levels were slightly decreased and hepatic thyroid hormone carrier MCT-8 mRNA levels were not modified. We propose that an enhanced liver thyroid hormone receptor in Lou/C may explain its unique resistance to obesity by increasing fatty acid oxidation and lowering liver oxidative phosphorylation stoichiometry at the translocation of reducing power into mitochondria.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2009
Language Anglais
Journal information "Journal of Biological Chemistry" - Vol. 284, no. 7, p. 4308-4316 (2009)
Peer reviewed yes
Publisher Amer Soc Biochemistry Molecular Biology Inc (Bethesda)
issn 0021-9258
e-issn 1083-351X
Affiliation UCL - MD/BICL - Département de biochimie et de biologie cellulaire
Links
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Bibliographic reference Taleux, Nellie ; Guigas, Bruno ; Dubouchaud, Herve ; Moreno, Maria ; Weitzel, Joachim M. ; et. al. High Expression of Thyroid Hormone Receptors and Mitochondrial Glycerol-3-phosphate Dehydrogenase in the Liver Is Linked to Enhanced Fatty Acid Oxidation in Lou/C, a Rat Strain Resistant to Obesity. In: Journal of Biological Chemistry, Vol. 284, no. 7, p. 4308-4316 (2009)
Permanent URL http://hdl.handle.net/2078.1/35825