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MRI of iron-oxide labelled transplanted hepatocytes in mice : effect of treatment with cyclophosphamide

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Leconte, Isabelle [UCL] Pallu, Stéphane [UCL] Abarca-Quinones, Jorge [UCL] Michoux, Nicolas [UCL] Peeters, Frank [UCL] Radermacher, Kim [UCL] Sempoux, Christine [UCL] Najimi, Mustapha [UCL] Sokal, Etienne [UCL] Van Beers, Bernard [UCL]

PURPOSE:: To assess if 1.5T MRI can be used to study the transport to the liver, the intrahepatic distribution and engraftment of iron-oxide labelled hepatocytes in cyclophosphamide-treated and untreated mice. MATERIALS AND METHODS:: Hepatocytes were isolated from C57bl/6 mice and were labelled with 1.63 mum iron-oxide particles. Seventeen mice were pretreated with cyclophosphamide to disrupt the sinusoidal endothelium and 15 were left untreated. Seven days after splenic injection of the labelled hepatocytes, T2*-weighted gradient-echo images at 1.5T were acquired. The hepatic transport, distribution and engraftment of the labelled hepatocytes were assessed with signal intensity (SI) and T2* measurements, electron paramagnetic resonance (EPR), texture analysis and histology. RESULTS:: Lower hepatic SI (P = 0.005), lower T2* (P = 0.033) and larger number of particles at histology (P = 0.006) suggested increased transport to the liver of labelled hepatocytes in cyclophosphamide-treated mice versus untreated mice. At histology, most particles were located in Kupffer cells. Particles distribution was heterogeneous. No difference between both groups was observed at texture analysis. CONCLUSION:: MRI is useful to assess the transport to the liver and intrahepatic distribution of transplanted labelled hepatocytes. The preferential location of iron-oxide particles within Kupffer cells after seven days limits the value of MRI for assessing hepatocyte engraftment. J. Magn. Reson. Imaging 2010;32:367-375. (c) 2010 Wiley-Liss, Inc.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article
Access type Accès libre
Publication date 2010
Language Anglais
Journal information "Journal of Magnetic Resonance Imaging" - Vol. 32, no. 2, p. 367-375 (2010)
Peer reviewed yes
Publisher JohnWiley & Sons, Inc. ((United States) Hoboken)
issn 1053-1807
e-issn 1522-2586
Publication status Publié
Affiliations UCL - SSS/IONS/NEUR - Clinical Neuroscience
UCL - SSS/IREC - Institut de recherche expérimentale et clinique
UCL - SSS/IONS - Institute of NeuroScience
UCL - SSS/LDRI - Louvain Drug Research Institute
UCL - SSS/IREC/PEDI - Pôle de Pédiatrie
UCL - SSS/IREC/GAEN - Pôle d'Hépato-gastro-entérologie
UCL - (SLuc) Service de radiologie
UCL - SSS/IREC/SLUC - Pôle St.-Luc
UCL - SSS/IREC/IMAG - Pôle d'imagerie médicale
UCL - (SLuc) Service d'anatomie pathologique
UCL - (SLuc) Service de gastro-entérologie et hépatologie pédiatrique
MESH Subject Spleen - pathology ; Microscopy, Electron, Transmission - methods ; Mice, Inbred C57BL ; Mice ; Male ; Magnetic Resonance Imaging - methods ; Liver - pathology ; Immunosuppressive Agents - pharmacology ; Hepatocytes - cytology ; Ferric Compounds - pharmacology ; Electron Spin Resonance Spectroscopy - methods ; Cyclophosphamide - pharmacology ; Cell Transplantation - methods ; Cell Survival ; Animals
Keywords Hepatocyte ; Cyclophosphamide ; MR imaging ; Transplantation ; Liver
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Bibliographic reference Leconte, Isabelle ; Pallu, Stéphane ; Abarca-Quinones, Jorge ; Michoux, Nicolas ; Peeters, Frank ; et. al. MRI of iron-oxide labelled transplanted hepatocytes in mice : effect of treatment with cyclophosphamide. In: Journal of Magnetic Resonance Imaging, Vol. 32, no. 2, p. 367-375 (2010)
Permanent URL http://hdl.handle.net/2078.1/33156