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A new LAGE-1 peptide recognized by cytolytic T lymphocytes on HLA-A68 tumors.

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Sun, Zhaojun [UCL] LETHE, Bernard [UCL] Zhang, Yi [UCL] Russo, Vincenzo Colau, Didier [UCL] Stroobant, Vincent [UCL] Boon-Falleur, Thierry [UCL] van der Bruggen, Pierre [UCL]

Antigens encoded by genes of the LAGE family, including LAGE-1 and NY-ESO-1, are of interest for cancer immunotherapy because they are tumor-specific and shared by tumors of different histological types. Several clinical trials are in progress with NY-ESO-1 peptides, protein, recombinant poxviruses, and dendritic cells pulsed with peptides. In this study, CD8 T lymphocytes from an individual without cancer were stimulated with dendritic cells infected with a recombinant avian poxvirus encoding a complete LAGE-1 protein. A CTL clone was isolated that recognized a new LAGE-1 peptide, ELVRRILSR, which corresponds to position 103-111 of the protein sequence. It is presented by HLA-A6801 molecules. When tumor cells expressing LAGE-1 were transfected with HLA-A68, they were lysed by the CTL clone, indicating that the peptide is processed in tumor cells. These results indicate that the LAGE-1.A68 peptide can be used for antitumoral vaccination. We observed also that specific T cells could be detected in a blood sample with a high sensitivity by using an A68/LAGE-1 fluorescent multimer.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2006
Journal information "Cancer Immunology, Immunotherapy : other biological response modifications" - Vol. 55, no. 6, p. 644-652 (2006)
Peer reviewed yes
Publisher Springer (Heidelberg)
issn 0340-7004
e-issn 1432-0851
Publication status Publié
Affiliation UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
MESH Subject Amino Acid Sequence ; Animals ; Antigen Presentation - immunology ; Antigens, Neoplasm - chemistry - genetics - immunology ; Antigens, Surface ; COS Cells ; Cercopithecus aethiops ; HLA Antigens - immunology ; Humans ; Melanoma - immunology ; Membrane Proteins - chemistry - genetics - immunology ; Molecular Sequence Data ; Peptides - genetics - immunology ; Polymerase Chain Reaction ; T-Lymphocytes, Cytotoxic - immunology ; Transfection
Keywords HLA-A68 ; LAGE-1 ; Peptide ; Poxvirus ; SNP ; Tumor
Links
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Bibliographic reference Sun, Zhaojun ; LETHE, Bernard ; Zhang, Yi ; Russo, Vincenzo ; Colau, Didier ; et. al. A new LAGE-1 peptide recognized by cytolytic T lymphocytes on HLA-A68 tumors.. In: Cancer Immunology, Immunotherapy : other biological response modifications, Vol. 55, no. 6, p. 644-652 (2006)
Permanent URL http://hdl.handle.net/2078.1/29467