Collet-Cassart, D
Limet, J N
Van Krieken, L
De Hertogh, René
[UCL]
In this latex immunoassay for human placental lactogen, microtiter plates are used as the reaction vessel and the absorbance at 405 nm is measured to quantify the reactions. This 30-min assay necessitates only one serum dilution and two pipetting steps. The calibration curve extends from 0.5 to 15 mg/L. CVs range from 4.2% to 7.0% for within-run determination and from 7.0% to 11.2% for between-run determinations. A correlation coefficient of 0.949 was obtained for 84 sera when the method was compared with a commercial radioimmunoassay.
Bibliographic reference |
Collet-Cassart, D ; Limet, J N ; Van Krieken, L ; De Hertogh, René. Turbidimetric latex immunoassay of placental lactogen on microtiter plates.. In: Clinical chemistry, Vol. 35, no. 1, p. 141-3 (1989) |
Permanent URL |
http://hdl.handle.net/2078.1/29400 |