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Impact of multispores in vitro subcultivation of Glomus sp. MUCL 43194 (DAOM 197198) on vegetative compatibility and genetic diversity detected by AFLP.

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Cardenas Flores, Antonio [UCL] Draye, Xavier [UCL] Bivort, Céline [UCL] Cranenbrouck, Sylvie [UCL] Declerck, Stephan [UCL]

Vegetative compatibility and amplified fragment length polymorphism (AFLP) genotyping of in vitro multispores clonal lineages, issued from the same ancestor culture of the arbuscular mycorrhizal fungal strain MUCL 43194 and subcultured several generations in different locations, was assessed. Vegetative compatibility was studied by confronting the germ tubes of two spores from the same or different clonal lineages and stained with nitrotetrazolium blue-Trypan blue and diamidinophenylindole to detect hyphal fusions and nuclei, respectively. Further AFLP analysis of single spores was performed to assess the genetic profile and Dice similarity between clonal lineages. Germ tubes of spores distant by as many as 69 generations were capable of fusing. The anastomosis frequencies averaged 69% between spores from the same clonal lineage, 57% between spores from different clonal lineages, and 0% between spores belonging to different strains. The AFLP patterns showed similarities averaging 92% within clonal lineages and 86% between clonal lineages. Each spore presented unique genotype and some of them shared more markers with spores from different lineages than within the same lineage. We showed that MUCL 43194 maintained self-recognition for long periods of subcultures in vitro and that spores involved in compatibility tests had different genotypes. Our findings suggest that MUCL 43194 maintains genetic diversity by means of anastomoses.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article
Access type Accès restreint
Publication date 2010
Language Anglais
Journal information "Mycorrhiza" - Vol. 20, no. 6, p. 415-425 (2010)
Peer reviewed yes
Publisher Springer ((Germany) Heidelberg)
issn 0940-6360
e-issn 1432-1890
Publication status Publié
Affiliation UCL - SST/ELI/ELIM - Applied Microbiology
MESH Subject Spores, Fungal - physiology ; Mycorrhizae - genetics - growth & development - physiology ; Glomeromycota - genetics - growth & development ; Genotype ; Genetic Variation ; Cell Culture Techniques ; Amplified Fragment Length Polymorphism Analysis
Links
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Bibliographic reference Cardenas Flores, Antonio ; Draye, Xavier ; Bivort, Céline ; Cranenbrouck, Sylvie ; Declerck, Stephan. Impact of multispores in vitro subcultivation of Glomus sp. MUCL 43194 (DAOM 197198) on vegetative compatibility and genetic diversity detected by AFLP.. In: Mycorrhiza, Vol. 20, no. 6, p. 415-425 (2010)
Permanent URL http://hdl.handle.net/2078.1/29037