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Transduction pathways regulating the trophic effects of Saccharomyces boulardii in rat intestinal mucosa.

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Buts, Jean-Paul [UCL] Dekeyser, Nadine [UCL]

(eng) Abstract Saccharomyces boulardii is a probiotic yeast that is widely prescribed in lyophilized form; it determines several effects in human and rat small intestine including endoluminal secretion of enzymes and of polyamines, stimulation of microvillous enzymes, of sIgA, increased production of the receptor for polymeric immunoglobulins by crypt cells, and enhanced d-glucose uptake. Aim. The objective of this study was to determine the pathway(s) by which these effects generated by the yeast are transduced into mucosal cells. Methods. Litters of six growing Wistar rats each were treated with S. boulardii (50 mug/gram body weight) or with saline between days 30 and 34 postpartum. For each animal, the cytosol was prepared from the whole mucosa after the fat cake was discarded. Several known intestinal substrates were immunoprecipitated and immunoblotted using specific antibodies recognizing the non-, mono-, or diphosphorylated forms of these substrates. The signals were detected using Echochemiluminoscence (ECL) and were measured by optodensitometry. Results. Treatment with S. boulardii markedly enhanced the RAS-GAP-RAF-ERK(1,2) pathway with participation of growth receptor bound 2 protein, SHC, SOS, and CRKII. Unit p85alpha of phosphatidylinositol 3 kinase, tested in its phosphorylated form, was also enhanced by the probiotic compared to control samples. In rats treated with an inhibitor of RAF-1 and of ERK(1,2) (PD098059) the expression of mucosal disaccharidases was inhibited by about 50%. Conclusion. The probiotic S. boulardii generates in vivo mitogen and metabolic signals that are transduced into intestinal mucosal cells, downstream from the apical membrane to the nuclei, using recruitment substrates and serine, threonine, or tyrosine kinases.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès libre
Publication date 2010
Language Anglais
Journal information "Scandinavian Journal of Gastroenterology" - Vol. 45, no. 2, p. 175-185 (2010)
Peer reviewed yes
Publisher Informa Healthcare ((United Kingdom) London)
issn 0036-5521
e-issn 1502-7708
Publication status Publié
Affiliations UCL - (SLuc) Service de pédiatrie spécialisée
UCL - SSS/IREC/PEDI - Pôle de Pédiatrie
MESH Subject Signal Transduction ; Saccharomyces - physiology ; Rats, Wistar ; Rats ; Proto-Oncogene Proteins c-crk - metabolism ; Protein-Serine-Threonine Kinases - metabolism ; Mitogen-Activated Protein Kinases - metabolism ; Jejunum - enzymology - microbiology ; Intestinal Mucosa - enzymology - metabolism - microbiology ; Disaccharidases - metabolism ; Animals
Keywords D-Glucose uptake ; Disaccharidases ; Polyamines ; Probiotic ; Saccharomyces boulardii ; Transduction pathways
Links
  1. Buts Jean-Paul, Bernasconi Paul, Saccharomyces boulardii: Basic Science and Clinical Applications in Gastroenterology, 10.1016/j.gtc.2005.05.009
  2. Buts Jean-Paul, Corthier Gérard, Delmee Michel, Saccharomyces boulardii for Clostridium difficile-Associated Enteropathies in Infants : , 10.1097/00005176-199305000-00013
  3. Guslandi Mario, Mezzi Gianni, Sorghi Massimo, Testoni Pier Alberto, 10.1023/a:1005588911207
  4. McFarland Lynne V., Meta-Analysis of Probiotics for the Prevention of Antibiotic Associated Diarrhea and the Treatment of Clostridium difficile Disease, 10.1111/j.1572-0241.2006.00465.x
  5. Surawicz CM, Gastroenterology, 96, 981 (1989)
  6. Szajewska H, Acta Paediatr, 94, 44 (2008)
  7. Buts Jean-Paul, Bernasconi Paul, Van Craynest Marie-Paule, Maldague Paul, De Meyer Roger, Response of Human and Rat Small Intestinal Mucosa to Oral Administration of Saccharomyces boulardii, 10.1203/00006450-198602000-00020
  8. Buts Jean-Paul, De Keyser Nadine, Stilmant Catherine, Sokal Etienne, Marandi Soheila, Saccharomyces boulardii Enhances N-Terminal Peptide Hydrolysis in Suckling Rat Small Intestine by Endoluminal Release of a Zinc-Binding Metalloprotease, 10.1203/00006450-200204000-00021
  9. Buts Jean-Paul, Stilmant Catherine, Bernasconi Paul, Neirinck Christiane, De Keyser Nadine, Characterization of α,α-trehalase released in the intestinal lumen by the probiotic Saccharomyces boulardii, 10.1080/00365520802308862
  10. Buts Jean-Paul, Bernasconi Paul, Vaerman Jean-Pierre, Dive Charles, Stimulation of secretory IgA and secretory component of immunoglobulins in small intestine of rats treated withSaccharomyces boulardii, 10.1007/bf01536771
  11. Buts J-P, De Keyser N, Marandi S, Hermans D, Sokal E M, Chae Y-H E, Lambotte L, Chanteux H, Tulkens P M, Saccharomyces boulardii upgrades cellular adaptation after proximal enterectomy in rats, 10.1136/gut.45.1.89
  12. Buts Jean-Paul, Keyser Nadine De, Raedemaeker Laurence De, Saccharomyces boulardii Enhances Rat Intestinal Enzyme Expression by Endoluminal Release of Polyamines, 10.1203/00006450-199410000-00019
  13. Buts Jean-Paul, De Keyser Nadine, Effects of Saccharomyces boulardii on Intestinal Mucosa, 10.1007/s10620-005-9016-x
  14. Buts JP, Pediatr Res, 60, 24 (2006)
  15. Marandi Soheila, De Keyser Nadine, Stilmant Catherine, Saliez Alain, Sokal Etienne Marc, Guiot Yves, Buts Jean-Paul, Ontogeny of MAP Kinases in Rat Small Intestine: Premature Stimulation by Insulin of BBM Hydrolases Is Regulated by ERKs but not by p-38 MAP Kinase, 10.1203/00006450-200208000-00009
  16. Messer M., Dahlqvist A., A one-step ultramicro method for the assay of intestinal disaccharidases, 10.1016/0003-2697(66)90280-6
  17. Lowry OH, J Biol Chem, 193, 265 (1951)
  18. Inukai Kouichi, Funaki Makoto, Ogihara Takehide, Katagiri Hideki, Kanda Akira, Anai Motonobu, Fukushima Yasushi, Hosaka Toshio, Suzuki Masakazu, Shin Bo-Chul, Takata Kuniaki, Yazaki Yoshio, Kikuchi Masatoshi, Oka Yoshitomo, Asano Tomoichiro, p85α Gene Generates Three Isoforms of Regulatory Subunit for Phosphatidylinositol 3-Kinase (PI 3-Kinase), p50α, p55α, and p85α, with Different PI 3-Kinase Activity Elevating Responses to Insulin, 10.1074/jbc.272.12.7873
  19. Skolnik EY, EMBO J, 12, 1929 (1993)
  20. van der Geer Peter, Wiley Sandra, Gish Gerald D., Pawson Tony, The Shc adaptor protein is highly phosphorylated at conserved, twin tyrosine residues (Y239/240) that mediate protein–protein interactions, 10.1016/s0960-9822(96)00748-8
  21. Khokhlatchev Andrei V, Canagarajah Bertram, Wilsbacher Julie, Robinson Megan, Atkinson Mark, Goldsmith Elizabeth, Cobb Melanie H, Phosphorylation of the MAP Kinase ERK2 Promotes Its Homodimerization and Nuclear Translocation, 10.1016/s0092-8674(00)81189-7
  22. Marandi S, Am J Physiol Gastrointest Liver Physiol, 43, G229 (2001)
  23. Gille H, EMBO J, 14, 951 (1995)
  24. Taylor L. K., Wang H. C., Erikson R. L., Newly identified stress-responsive protein kinases, Krs-1 and Krs-2., 10.1073/pnas.93.19.10099
  25. Sung CK, J Biol Chem, 269, 12503 (1994)
  26. Wong Gail, Müller Oliver, Clark Robin, Conroy Leah, Moran Michael F., Polakis Paul, McCormick Frank, Molecular cloning and nucleic acid binding properties of the GAP-associated tyrosine phosphoprotein p62, 10.1016/0092-8674(92)90455-l
  27. Weng Qing-Ping, Kozlowski Mark, Belham Christopher, Zhang Aihua, Comb Michael J., Avruch Joseph, Regulation of the p70 S6 Kinase by Phosphorylationin Vivo : ANALYSIS USING SITE-SPECIFIC ANTI-PHOSPHOPEPTIDE ANTIBODIES, 10.1074/jbc.273.26.16621
  28. Beg AA, Oncogene, 9, 1487 (1994)
  29. Dahan S., Dalmasso G., Imbert V., Peyron J.-F., Rampal P., Czerucka D., Saccharomyces boulardii Interferes with Enterohemorrhagic Escherichia coli-Induced Signaling Pathways in T84 Cells, 10.1128/iai.71.2.766-773.2003
  30. POTHOULAKIS C., Review article: anti-inflammatory mechanisms of action ofSaccharomyces boulardii, 10.1111/j.1365-2036.2009.04102.x
  31. Buts Jean-Paul, Dekeyser Nadine, Stilmant Catherine, Delem Emilie, Smets Francoise, Sokal Etienne, Saccharomyces boulardii Produces in Rat Small Intestine a Novel Protein Phosphatase that Inhibits Escherichia coli Endotoxin by Dephosphorylation, 10.1203/01.pdr.0000220322.31940.29
Bibliographic reference Buts, Jean-Paul ; Dekeyser, Nadine. Transduction pathways regulating the trophic effects of Saccharomyces boulardii in rat intestinal mucosa.. In: Scandinavian Journal of Gastroenterology, Vol. 45, no. 2, p. 175-185 (2010)
Permanent URL http://hdl.handle.net/2078.1/28816