User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Molecular characterization of Clostridium difficile clinical isolates in a geriatric hospital.

Primary tabs

Poilane, Isabelle Humeniuk-Ainouz, Christel Durand, Isabelle Janoir, Claire Cruaud, Philippe Delmée, Michel [UCL] Collignon, Anne

The discriminatory potential of a combination of various typing methods was evaluated on a set of 21 Clostridium difficile isolates obtained from symptomatic patients hospitalized in a geriatric unit and 7 non-toxigenic isolates from the same hospital. Isolates were firstly serotyped and toxinotyped. Of the 28 isolates, 19 belonged to serogroup A. PCR-ribotyping and PCR-RFLP on the fliC and slpA genes were then applied to these 19 isolates. The results suggest that the combination of PCR-ribotyping with PCR-RFLP analysis of slpA could be more discriminatory and suitable for studying C. difficile epidemiology.

Document type Article de périodique (Journal article) – Comparative Study, Evaluation Studies, Journal Article
Access type Accès restreint
Publication date 2007
Journal information "Journal of medical microbiology" - Vol. 56, no. Pt 3, p. 386-90 (2007)
Peer reviewed yes
issn 0022-2615
Publication status Publié
Affiliations UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
UCL - (SLuc) Service de microbiologie
MESH Subject Bacterial Proteins - genetics ; Bacterial Toxins - analysis ; Bacterial Typing Techniques - methods ; Clostridium difficile - classification - genetics - isolation & purification ; Cross Infection - microbiology ; Enterocolitis, Pseudomembranous - microbiology ; Epidemiology, Molecular - methods ; France ; Genotype ; Geriatrics ; Hospitals, Special ; Humans ; Phenotype ; Polymorphism, Restriction Fragment Length ; Ribotyping ; Serotyping
Links
Bibliographic reference Poilane, Isabelle ; Humeniuk-Ainouz, Christel ; Durand, Isabelle ; Janoir, Claire ; Cruaud, Philippe ; et. al. Molecular characterization of Clostridium difficile clinical isolates in a geriatric hospital.. In: Journal of medical microbiology, Vol. 56, no. Pt 3, p. 386-90 (2007)
Permanent URL http://hdl.handle.net/2078.1/27755