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Aerobic growth of Escherichia coli with 2,4,6-trinitrotoluene (TNT) as the sole nitrogen source and evidence of TNT denitration by whole cells and cell-free extracts.

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Stenuit, Benoît [UCL] Agathos, Spiros N. Eyers, Laurent [UCL] Rozenberg, Raoul [UCL] Habib Jiwan, Jean-Louis [UCL]

Escherichia coli grew aerobically with 2,4,6-trinitrotoluene (TNT) as sole nitrogen source and caused TNT's partial denitration. This reaction was enhanced in nongrowing cell suspensions with 0.516 mol nitrite released per mol TNT. Cell extracts denitrated TNT in the presence of NAD(P)H. Isomers of amino-dimethyl-tetranitrobiphenyl were detected and confirmed with U-15N-labeled TNT.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès libre
Publication date 2006
Journal information "Applied and environmental microbiology" - Vol. 72, no. 12, p. 7945-8 (2006)
Peer reviewed yes
issn 0099-2240
Publication status Publié
Affiliations UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
UCL - SC/CHIM - Département de chimie
MESH Subject Aerobiosis ; Biodegradation, Environmental ; Culture Media ; Escherichia coli - cytology - growth & development - metabolism ; Kinetics ; Nitrites - metabolism ; Nitrogen ; Trinitrotoluene - metabolism
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Bibliographic reference Stenuit, Benoît ; Agathos, Spiros N. ; Eyers, Laurent ; Rozenberg, Raoul ; Habib Jiwan, Jean-Louis. Aerobic growth of Escherichia coli with 2,4,6-trinitrotoluene (TNT) as the sole nitrogen source and evidence of TNT denitration by whole cells and cell-free extracts.. In: Applied and environmental microbiology, Vol. 72, no. 12, p. 7945-8 (2006)
Permanent URL http://hdl.handle.net/2078.1/27671