Gbenle, G O
Opperdoes, Frederik
[UCL]
Van Roy, Joris
[UCL]
We have characterized a 3'-nucleotidase activity of T. brucei. The enzyme has a pH optimum of 8.7, is inactivated by chelating agents and stimulated by divalent cations. It is inhibited by Zn2+, Mn2+, pyrophosphate and the trypanocidal drug suramin for which it has a Ki of 3 microM. From cell fractionation experiments it is concluded that the enzyme is located in the plasma membrane. Alkaline 3'-endoribonuclease is also located in the plasma membrane of T. brucei and this activity shares a great number of properties with the 3'-nucleotidase activity, including its sensitivity to suramin. The possibility that both 3'-nucleotidase and endonuclease activities are catalyzed by the same enzyme cannot be excluded.
Bibliographic reference |
Gbenle, G O ; Opperdoes, Frederik ; Van Roy, Joris. Localization of 3'-nucleotidase and calcium-dependent endoribonuclease in the plasma-membrane of Trypanosoma brucei.. In: Acta tropica, Vol. 43, no. 4, p. 295-305 (1986) |
Permanent URL |
http://hdl.handle.net/2078.1/27042 |