Safety of Lavender Oil-Loaded Niosomes for In Vitro Culture and Biomedical Applications.

de Miranda Vasconcellos Vilela, Janice [UCL] Moghassemi, Saeid [UCL] Dadashzadeh, Arezoo [UCL] Dolmans, Marie-Madeleine [UCL] Azevedo, Ricardo B Andrade Amorim, Christiani [UCL]

(eng) (1) BACKGROUND: Essential oils have long been used as therapeutic agents. Lavender (Lavandula angustifolia) oil (LO) is an antispasmodic, anticonvulsant, relaxant, painkilling, and antimicrobial essential oil investigated as a natural substance for biomedical therapies. Nanoparticles have shown significant promise in improving drug delivery and efficacy. Considering these benefits, the aim of this study was to evaluate the toxicity of LO and lavender oil niosomes (LONs) in stem cells and myofibroblast models cultured in vitro. (2) METHODS: Adipose tissue-derived stem cells and myometrial cells were cultured with LO or LONs at different concentrations (0, 0.016%, 0.031%, and 0.063%) and toxicity was evaluated with PrestoBlue™ and live/dead assay using calcein and ethidium homodimer. (3) RESULTS: Cell viability was similar to controls in all groups, except in 0.063% LO for myometrial cells, which showed lower viability than the control medium. (4) CONCLUSION: These results suggest that both LO and LONs are safe for cell culture and may be used for pharmaceutical and biomedical therapies in future applications in regenerative medicine., NCT01721044, NCT01721057, NCT01711359 and NCT01885078.