Steuve, Séverine
Devosse, Thalie
Lauwers, Elsa
Vanderwinden, Jean-Marie
André, Bruno
Courtoy, Pierre J.
[UCL]
Pirson, Isabelle
Rhophilin-2 or p76(RBE), a protein whose expression is induced by the cyclic AMP pathway in thyrocytes, contains several protein-protein interaction domains including HR-1, Bro1 and PDZ domains, and is a partner of RhoB in its GTP-bound form (Eur J Biochem, 269(24): 6241-9, 2002). We here define its subcellular localization and dissect the significance of its domains. By subcellular fractionation and colocalization experiments, rhophilin-2 is recruited to subcellular organelles by activated RhoB-GTP. As for its yeast homologue, Npi3/Bro1p, the Bro1 domain of rhophilin-2 is necessary to its recruitment to the vesicular structures, which are not labeled for EEA1 nor Lamp1, but well with the late endosome marker CD63.
Bibliographic reference |
Steuve, Séverine ; Devosse, Thalie ; Lauwers, Elsa ; Vanderwinden, Jean-Marie ; André, Bruno ; et. al. Rhophilin-2 is targeted to late-endosomal structures of the vesicular machinery in the presence of activated RhoB.. In: Experimental cell research, Vol. 312, no. 20, p. 3981-3989 (2006) |
Permanent URL |
http://hdl.handle.net/2078.1/25891 |