Ovarian tissue cryopreservation and transplantation is a widely applied approach to preserve fertility in cancer patients, but little is known about the effects of ovarian tissue transportation. The goal of this study was to analyze different parameters of media used for ovarian tissue storage. Cow ovaries were obtained from a local abattoir and divided into 1x1 cm fragments, then immersed in either 0.9% NaCl solution, IVF medium or L-15 medium for 1, 4 or 24 hours at 4°C. After each period, pH, lactate dehydrogenase (LDH) activity, reactive oxygen species (ROS), glucose, lactate and pyruvate were evaluated in the media. Results showed a lower pH in NaCl at all timepoints compared to IVF and L-15 media. LDH activity progressively increased in each medium and was significantly lower in NaCl than in IVF/L-15 at 1 and 4 hours, indicating lower cell death rates in NaCl. A small but significant increase in ROS production was observed over time, higher in NaCl than IVF and L-15. There was no significant difference in glucose, but a significant decrease in pyruvate concentration in L-15 and increase in lactate production in all media. Results suggest there is important metabolic activity in the ovary during transportation for up to 24h at 4°C. In conclusion, our preliminary data shed new light on the impact of transportation media, highlighting the importance of understanding more about this period and its impact on graft survival.
de Miranda Vasconcellos Vilela, Janice ; et. al. Impact of cold ischemia time in different media for ovarian transportation: preliminary results.The 6th World Congress of the International Society for Fertility Preservation (ISFP) (New York, USA, du 14/11/2019 au 16/11/2019). In: Abstracts from the 2019 ISFP meeting in NYC, Vol. 37, no.4, p. 987-1015 (2020)
