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Gas chromatographic and mass fragmentographic assays of carcinogenic polycyclic hydrocarbon epoxide hydratase activity.

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Bettencourt, A Lhoëst, Georges [UCL] Roberfroid, Marcel [UCL] Mercier, Michel [UCL]

A specific and very sensitive procedure for the determination of epoxide hydratase activity in hepatic microsomes is described. Any polycyclic hydrocarbon epoxide can be used as a substrate; in this study, benzo(a)anthracene-5,6-oxide, benzo(a)pyrene-4,5-oxide and 3-methylcholanthrene-11,12-oxide were utilized. The corresponding trans-diols formed during incubation are separated and evaluated using either an electron-capture gas chromatographic method for the determination of their chloromethyldimethylsilylated derivatives or gas chromatographic-mass fragmentographic measurement of their trimethylsilylated derivatives. Concentrations as low as 1 ng per millilitre of incubation mixture can be estimated.

Document type Article de périodique (Journal article) – Journal Article
Publication date 1977
Journal information "Journal of chromatography" - Vol. 134, no. 2, p. 323-30 (1977)
Peer reviewed yes
issn 0021-9673
Publication status Publié
Affiliation UCL
MESH Subject Animals ; Benz(a)Anthracenes ; Benzopyrenes ; Carcinogens ; Chromatography, Gas - methods ; Epoxide Hydrolases - analysis ; Gas Chromatography-Mass Spectrometry - methods ; Hydro-Lyases - analysis ; Kinetics ; Male ; Methylcholanthrene ; Microsomes, Liver - enzymology ; Oxides ; Polycyclic Compounds ; Rats
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Bibliographic reference Bettencourt, A ; Lhoëst, Georges ; Roberfroid, Marcel ; Mercier, Michel. Gas chromatographic and mass fragmentographic assays of carcinogenic polycyclic hydrocarbon epoxide hydratase activity.. In: Journal of chromatography, Vol. 134, no. 2, p. 323-30 (1977)
Permanent URL http://hdl.handle.net/2078.1/25173