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Separate isolation of cells from nodules and surrounding parenchyma of the same precancerous rat liver: biochemical and cytochemical characterization.

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de Gerlache, J Lans, M. Taper, Henryk [UCL] Roberfroid, Marcel [UCL]

Various enzyme and metabolic alterations have been observed in the hyperplastic nodules which appear during the hepatocarcinogenesis. These alterations have been mainly specified by histochemical observations. In this report, a technique of hepatocyte isolation is described which enables the separation of 2 cellular fractions, respectively, from the nodules and from the surrounding parenchyma of the same liver of a rat previously treated with a hepatocarcinogen. Such a technique allowed parallel analysis of both cellular populations by biochemical and cytochemical techniques.

Document type Article de périodique (Journal article) – In Vitro, Journal Article
Publication date 1980
Journal information "Toxicology" - Vol. 18, no. 3, p. 225-32 (1980)
Peer reviewed yes
issn 0300-483X
Publication status Publié
Affiliation UCL
MESH Subject Animals ; Cell Separation ; Histocytochemistry ; Liver - cytology - ultrastructure ; Liver Glycogen - metabolism ; Liver Neoplasms - metabolism ; Male ; Precancerous Conditions - metabolism ; Rats ; Subcellular Fractions - metabolism
Links
Bibliographic reference de Gerlache, J ; Lans, M. ; Taper, Henryk ; Roberfroid, Marcel. Separate isolation of cells from nodules and surrounding parenchyma of the same precancerous rat liver: biochemical and cytochemical characterization.. In: Toxicology, Vol. 18, no. 3, p. 225-32 (1980)
Permanent URL http://hdl.handle.net/2078.1/25142