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Involvement of lipogenesis in the lower VLDL secretion induced by oligofructose in rats

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Kok, Nadine [UCL] Roberfroid, Marcel [UCL] Robert, Annie [UCL] Delzenne, Nathalie M. [UCL]

Dietary supplementation with oligofructose (OFS; 100 g/kg), a non-digestible oligomer of beta-D-fructose, decreases serum triacylglycerols in serum and VLDL of rats. In order to investigate the role of hepatic metabolism in the hypolipidaemic effect of OFS, male Wistar rats were fed on a standard diet with or without 100 g Raftilose P95/kg as OFS source for 30 d. OFS feeding (1) significantly decreased triacylglycerol and phospholipid concentrations in both blood and liver, (2) increased the glycerol-3-phosphate liver content but decreased the hepatic activity of glycerol-3-phosphate acyltransferase (EC 2.3.1.15), suggesting a decrease in acylglycerol synthesis, (3) did not affect the blood non-esterified fatty acid concentrations, but (4) reduced by 54% the capacity of isolated hepatocytes to synthesize and secrete triacylglycerols from labelled acetate; the activity of fatty acid synthase, a key lipogenic enzyme was also significantly decreased. These findings suggest that OFS decreases serum triacylglycerols by reducing de novo fatty acid synthesis in the liver; the lower insulin level in the serum of OFS-fed rats could explain, at least partly, the metabolic effect induced by such non-digestible carbohydrates.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1996
Language Anglais
Journal information "The British Journal of Nutrition : an international journal of nutritional science" - Vol. 76, no. 6, p. 881-890 (1996)
Peer reviewed yes
Publisher Cambridge University Press ((United Kingdom) Cambridge)
issn 0007-1145
e-issn 1475-2662
Publication status Publié
Affiliation UCL - MD/FARM - Ecole de pharmacie
MESH Subject Animals ; Fatty Acids - metabolism ; Fructose - administration & dosage ; Glycerol-3-Phosphate O-Acyltransferase - metabolism ; Lipids - biosynthesis ; Lipoproteins, VLDL - secretion ; Liver - enzymology - metabolism ; Male ; Oligosaccharides - administration & dosage ; Phospholipids - metabolism ; Rats ; Rats, Wistar ; Triglycerides - metabolism
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Bibliographic reference Kok, Nadine ; Roberfroid, Marcel ; Robert, Annie ; Delzenne, Nathalie M.. Involvement of lipogenesis in the lower VLDL secretion induced by oligofructose in rats. In: The British Journal of Nutrition : an international journal of nutritional science, Vol. 76, no. 6, p. 881-890 (1996)
Permanent URL http://hdl.handle.net/2078.1/25067