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Immunoassay by particle counting for coagulation testing: application to the determination of protein C.

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Poncelet, S. Lavenne, E. Limet, J N Collet-Cassart, D

Latex particles coated with F(ab)'2 fragments of anti-protein C IgG antibodies are agglutinated by protein C, and the quantity of particles agglutinated is proportional to the concentration of protein C. The reaction can be quantitated by optical particle counting. Based on this system, we designed an immunoassay for protein C. Precision measured at low, medium and high levels of protein C varied from 3.3% to 13.7%. Specificity was evaluated by dilution recovery. A correlation coefficient of r = 0.959 was found when the new method was compared with a chromogenic technique on 131 plasmas.

Document type Article de périodique (Journal article) – Comparative Study, Journal Article
Publication date 1990
Journal information "Journal of clinical chemistry and clinical biochemistry. Zeitschrift für klinische Chemie und klinische Biochemie" - Vol. 28, no. 3, p. 181-3 (1990)
issn 0340-076X
Publication status Publié
Affiliation UCL
MESH Subject Animals ; Blood Coagulation Tests - methods ; Calibration ; Cattle ; Chromogenic Compounds - metabolism ; Humans ; Immunoassay - methods ; Protein C - analysis
Links
Bibliographic reference Poncelet, S. ; Lavenne, E. ; Limet, J N ; Collet-Cassart, D. Immunoassay by particle counting for coagulation testing: application to the determination of protein C.. In: Journal of clinical chemistry and clinical biochemistry. Zeitschrift für klinische Chemie und klinische Biochemie, Vol. 28, no. 3, p. 181-3 (1990)
Permanent URL http://hdl.handle.net/2078.1/24767