User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Detection of inner ear disease autoantibodies by immunoblotting

Primary tabs

Cao, Ming-Yu [UCL] Gersdorff, Michel [UCL] Deggouj, Naima [UCL] Warny, Michel [UCL] Tomasi, Jean-Paul [UCL]

To define further the character of autoantibodies against the inner ear in patients with inner ear disease, Autoantibodies in sera from 82 patients with inner ear disease were investigated by immunoblotting. The inner ear antigens were extracted from Hartley guinea pigs. Brain, kidney, lung, heart and liver extracts were also prepared. Antibodies against the inner ear were found in 32 of 82 (39%) patients with inner ear disease. These sera reacted with the 30 and 58 kDa bands of the inner ear extracts. The 30 kDa band was detected in sera from patients with various inner ear diseases, while the 58 kDa band reacted with sera of patients with idiopathic progressive sensorineural hearing loss. Only two of the 52 normal control sera had a very faint band at 30 kDa. Sixteen of 32 positive sera were then used to probe Western blots of the brain, kidney, lung heart and liver extracts. The 58 kDa band was also found in the protein extracts of the brain, the lung, and the liver. On the other hand, preliminary purification of the 30 and 58 kDa proteins from the inner ear extracts were achieved by anion exchange chromatography. These results show that antibodies in sera from patients with inner ear disease reacted with at least two polypeptide bands (30 and 58 kDa) of guinea pig inner ear extracts, and the 58 kDa antigenic epitope was not cochlea specific.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article
Access type Accès restreint
Publication date 1994
Language Anglais
Journal information "Molecular and Cellular Biochemistry : an international journal for chemical biology in health and disease" - Vol. 146, no. 2, p. 157-163 (1994)
Peer reviewed yes
Publisher Springer New York LLC (New York)
issn 0300-8177
e-issn 1573-4919
Publication status Publié
Affiliations UCL - MD/NOPS - Département de neurologie et de psychiatrie
UCL - (SLuc) Service d'oto-rhino-laryngologie
UCL - (SLuc) Service de microbiologie
MESH Subject Adolescent ; Adult ; Aged ; Animals ; Antigens - chemistry - isolation & purification ; Autoantibodies - blood ; Blotting, Western ; Case-Control Studies ; Ear, Inner - immunology ; Female ; Guinea Pigs ; Hearing Loss, Sensorineural - immunology ; Hearing Loss, Sudden - immunology ; Humans ; Labyrinth Diseases - immunology ; Male ; Meniere Disease - immunology ; Middle Aged ; Molecular Weight ; Otosclerosis - immunology ; Proteins - chemistry - immunology - isolation & purification
Keywords Cochlea ; Hearing loss ; Autoantibody ; Inner ear antigen ; Western blot ; Chromatography
Links
  1. McCabe BF: Autoimmune sensorineural hearing loss. Ann Otol Rhinol Laryngol 88: 585?590, 1979
  2. McCabe BF and McCormick KJ: Tests for autoimmune disease in otology. Am J Otol 5: 447?449, 1984
  3. Hughes GB, Kinney SE, Barna BP, Calabrese LH: Practical versus theoretical management of autoimmune inner ear disease. Laryngoscope 94: 758?766, 1984
  4. Evans KL, Baldwin DL, Bainbridge DM, Morrison AW: Immune status in patients with Meniere's disease. Arch Otorhinolaryngoi 245: 287?292, 1988
  5. Hughes GB, Kinney SE, Barna BP, Calabrese HL: Autoimmune reactivity in Meniere's disease: A preliminary report. Laryngoscope 93: 410?417, 1983
  6. Hughes GB, Barna BP, Kinney SE, Calabrese LH, Nalepa NL: Predictive value of laboratory tests in ?autoimmune? inner ear disease: preliminary report. Laryngoscope 96: 502?505, 1986
  7. Arnold W, Pfaltz CR: Critical evaluation of the immunofluorescence microscopic test for identification of serum antibodies against human inner ear tissue. Acta Otolaryngol (Stockh) 103: 373?378, 1987
  8. Gersdorff M, Cao MY, Tomasi J-P, Deggouj N: Maladies Auto-immunes de L'Oreille. Etude des Auto-Anticorps Anticochlec. Rapport Preliminaire. Revue de Laryngologie Otologie Rhinologie 115: 163?167, 1994
  9. Veldman JE, Roord JJ, O'Connor AF, Shea JJ: Autoimmunity and inner ear disorders: An immune-complex mediated sensorineural hearing loss. Laryngoscope 94: 501?507, 1984
  10. Luetje CM: Theoretical and practical implications for plasmaphoresis in autoimmune inner ear disease. Laryngoscope 99: 1137?1146, 1989
  11. Yoo TJ, Tomoda K, Stuart JM, Kang AH, Townes AS: Type II collagen-induced autoimmune otospongiosis. A preliminary report. Ann Otol Rhinol Laryngol 92: 103?108, 1983
  12. Yoo TJ: Etiopathogenesis of otosclerosis: A hypothesis. Ann Otol Rhinol Laryngol 93(1): 28?33, 1984
  13. Stephens SD, Luxon L, Hincholiffe R: Immunological disorder and auditory lesions. Audiology 21(2): 128?148, 1982
  14. Joliat T, Seyer J, Bernstein J, Krug M, Ye XJ, Cho JS, Fujiyoshi T, Yoo TJ: Antibodies against a 30 kilodalton cochlear protein and type II and IX collagens in the serum of patients with inner ear disease. Ann Otol Rhinol Laryngol 101: 1000?1006, 1992
  15. Arnold W, Pfaltz R, Altermatt H-J: Evidence of serum antibodies against inner ear tissues in the blood of patients with certain sensorineural hearing disorders. Acta Otolaryngol (Stockh) 99: 437?444, 1985
  16. Harris JP: Experimental autoimmune sensorineural hearing loss. Laryngoscope 97: 63?76, 1987
  17. Yamanobe S, Harris JP: Spontaneous remission experimental autoimmune labyrinthitis. Ann Otol Rhinol laryngol 101: 1007?1014, 1992
  18. Harris JP, Sharp PA: Inner ear autoantibodies in patients with rapidly progressive sensorineural hearing loss. Laryngoscope 100: 516?524, 1990
  19. Soliman AM: Experimental autoimmune inner ear disease. Laryngoscope 99: 188?93, 1989
  20. Orozco CR, Niparko JK, Richardson BC, Dolan DF, Ptok MU, Altschuler RA: Experimental model of immune-medicated hearing loss using cross-species immunization. Laryngoscope 100: 941?7, 1990
  21. Yoo TJ, Yazawa Y, Floyd R: Type II collagen-induced autoimmune endolymphatic hydrops in guinea pig. Science 222: 65?7, 1983
  22. Yoo TJ, Tomoda K, Hernandez AD: Type II collagen-induced autoimmune inner ear lesions in guinea pigs. Ann Otol Rhinol Laryngol 93(suppl 113): 3?5, 1984
  23. Veldman JE, Hanada T, Meeuwsen F: Diagnostic and therapeutic dilemmas in rapidly progressive sensorineural hearing loss and sudden deafness. Acta Otolaryngol (Stockh) 113: 303?306, 1993
  24. Cao MY, Wong Y, Wang J, Dai SH: Scanning electron microscope specimen preparation of guinea pig vestibular organ. Acta Academiae Medicinae Nanjing 8(4): 341?342, 1988
  25. Suzuki H, Terada T: Removal of dodecyl sulfate from protein solution. Anal Biochem 172: 259?263, 1988
  26. Morrissey JH: Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem 117: 307?310, 1981
  27. Harris JP: Immunology of the inner ear: Response of the inner ear to antigen challenge. Otolaryngol Head Neck Surg 91: 18?23, 1983
  28. Harris JP: Immunology of the inner ear: Evidence of local antibody production. Ann Otol Rhinol Laryngol 93: 157?162, 1984
  29. Mogi G, Lim DJ, Watanabe N: Immunologic study on the inner ear: Immunoglobulins in perilymph. Arch Otolaryngol 108: 270?275, 1982
  30. Tomiyama S, Harris JP: The role of the endolymphatic sac in inner ear immunity. Acta Otolaryngol (Stockh) 103: 182?188, 1987
  31. Soliman M: A comparative immunofluorescent study of fixed decalcified tissue and frozen non-decalcified tissue from the guinea pig cochlea. Arch Otorhinolaryngol 244: 337?341, 1988
Bibliographic reference Cao, Ming-Yu ; Gersdorff, Michel ; Deggouj, Naima ; Warny, Michel ; Tomasi, Jean-Paul. Detection of inner ear disease autoantibodies by immunoblotting. In: Molecular and Cellular Biochemistry : an international journal for chemical biology in health and disease, Vol. 146, no. 2, p. 157-163 (1994)
Permanent URL http://hdl.handle.net/2078.1/24413