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31P NMR saturation transfer study of the creatine kinase reaction in human skeletal muscle at rest and during exercise

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Goudemant, Jean-François [UCL] Francaux, Marc [UCL] Mottet, Isabelle [UCL] Demeure, Roger [UCL] Sibomana, Merence [UCL] Sturbois, Xavier [UCL]

The creatine kinase reaction has been studied by 31P NMR in exercising human calf muscle. Quantitative analysis of high energy phosphates and saturation transfer study of the creatine kinase flux in the direction of ATP synthesis (Vfor) were performed at rest and during exercise. As expected, exercise induced a [PCr] decrease (from 28.5 +/- 0.9 to 21.9 +/- 1.5 mM, P < 0.01) matched by a Pi increase (from 4.5 +/- 0.2 to 8.9 +/- 1.8 mM, P = 0.06). pHi and [ATP] remained unchanged. Vfor did not change from rest (12.4 +/- 0.9 mM s(-1)) to moderate exercise and decreased at the highest exercise level (8.4 +/- 1.4 mM s(-1), P = 0.006). This observation differs from the prediction of the creatine kinase rate equation, showing an increase in the flux with exercise intensity. Computations suggest that this discrepancy arises from metabolite compartmentalization and/or from the reaction kinetics of a dead end complex stabilized by planar anions.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 1997
Journal information "Magnetic Resonance in Medicine" - Vol. 37, no. 5, p. 744-753 (1997)
Peer reviewed yes
Publisher JohnWiley & Sons, Inc. (Hoboken)
issn 0740-3194
e-issn 1522-2594
Publication status Publié
Affiliation UCL - MD/IEPR - Institut d'éducation physique et de réadaptation
MESH Subject Adult ; Creatine Kinase - metabolism ; Exercise ; Humans ; Hydrogen-Ion Concentration ; Leg ; Magnetic Resonance Spectroscopy ; Male ; Muscle, Skeletal - enzymology ; Rest
Keywords Energy metabolism ; Creatine kinase ; Human skeletal muscle
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Bibliographic reference Goudemant, Jean-François ; Francaux, Marc ; Mottet, Isabelle ; Demeure, Roger ; Sibomana, Merence ; et. al. 31P NMR saturation transfer study of the creatine kinase reaction in human skeletal muscle at rest and during exercise. In: Magnetic Resonance in Medicine, Vol. 37, no. 5, p. 744-753 (1997)
Permanent URL http://hdl.handle.net/2078.1/24273