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Differential transfer dynamics of pAW63 plasmid among members of the Bacillus cereus group in food microcosms.

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Modrie, Pauline [UCL] Beuls, Elise [UCL] Mahillon, Jacques [UCL]

Abstract Aim: To assess the dynamics of plasmid transfer between Bacillus thuringiensis and B. cereus in various food microcosms using the B. thuringiensis pAW63 and Staphylococcus aureus pUB110 plasmids as models. Methods and Results: The conjugative behaviour of pAW63, which resembles the B. anthracis virulence plasmid pXO2, and the mobilization of pUB110 were investigated using kinetics studies performed in reference LB (lysogeny broth) medium, full-cream and skimmed milks, soya milk and rice milk. Transfers of pAW63 and pUB110 were found to occur in the five tested media, with higher frequencies observed in food matrices, most notably in full-cream milk, skimmed milk and soya milk, where the mean transfer frequencies reached 10(-3) transconjugants per recipient cell. The most notable observations were that the higher transfer frequencies obtained in foodstuffs compared to those observed in LB were because of an earlier onset of conjugation in combination with a higher transfer rate and/or a longer mating period. Conclusion: These results indicate that not only the potential for plasmid transfer but also the overall timing of conjugation is affected by each of these food matrices. Significance and Impact of the Study: This new approach to study plasmid transfer provides insights for a better understanding of conjugation in food microcosms from both animal and vegetable origins among members of the B. cereus group.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2010
Language Anglais
Journal information "Journal of applied microbiology" - Vol. 108, no. 3, p. 888-897 (2010)
Peer reviewed yes
issn 1364-5072
e-issn 1365-2672
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Soybeans - microbiology ; Plasmids - genetics ; Oryza sativa - microbiology ; Milk - microbiology ; Food Microbiology ; Culture Media ; Conjugation, Genetic ; Bacillus thuringiensis - genetics ; Bacillus cereus - genetics ; Animals
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Bibliographic reference Modrie, Pauline ; Beuls, Elise ; Mahillon, Jacques. Differential transfer dynamics of pAW63 plasmid among members of the Bacillus cereus group in food microcosms.. In: Journal of applied microbiology, Vol. 108, no. 3, p. 888-897 (2010)
Permanent URL http://hdl.handle.net/2078.1/23837