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Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal.

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Mignolet, Johann [UCL] Cerckel, Guillaume [UCL] Damoczi, Julien [UCL] Ledesma-García, Laura [UCL] Sass, Andrea Hols, Pascal [UCL]

Constantly surrounded by kin or alien organisms in nature, eukaryotes and prokaryotes developed various communication systems to coordinate adaptive multi-entity behavior. In complex and overcrowded environments, they require to discriminate relevant signals in a myriad of pheromones to execute appropriate responses. In the human gut commensal , the cytoplasmic Rgg/RNPP regulator ComR couples competence to bacteriocin-mediated predation. Here, we describe a paralogous sensor duo, ScuR and SarF, which circumvents ComR in order to disconnect these two physiological processes. We highlighted the recurring role of Rgg/RNPP in the production of antimicrobials and designed a robust genetic screen to unveil potent/optimized peptide pheromones. Further mutational and biochemical analyses dissected the modifiable selectivity toward their pheromone and operating sequences at the subtle molecular level. Additionally, our results highlight how we might mobilize antimicrobial molecules while silencing competence in endogenous populations of human microflora and temper gut disorders provoked by bacterial pathogens.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2019
Language Anglais
Journal information "eLife" - Vol. 8 (2019)
Peer reviewed yes
e-issn 2050-084X
Publication status Publié
Affiliation UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
Keywords RNPP ; Streptococcus salivarius ; bacteriocin ; biochemistry ; cell-cell communication ; chemical biology ; competence ; infectious disease ; microbiology ; predation
Links
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Bibliographic reference Mignolet, Johann ; Cerckel, Guillaume ; Damoczi, Julien ; Ledesma-García, Laura ; Sass, Andrea ; et. al. Subtle selectivity in a pheromone sensor triumvirate desynchronizes competence and predation in a human gut commensal.. In: eLife, Vol. 8 (2019)
Permanent URL http://hdl.handle.net/2078.1/218934