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Requirement for NF-κB in maintenance of molecular and behavioral circadian rhythms in mice.

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Hong, Hee-Kyung Maury, Eléonore [UCL] Ramsey, Kathryn Moynihan Perelis, Mark Marcheva, Biliana Bass, Joseph

The mammalian circadian clock is encoded by an autoregulatory transcription feedback loop that drives rhythmic behavior and gene expression in the brain and peripheral tissues. Transcriptomic analyses indicate cell type-specific effects of circadian cycles on rhythmic physiology, although how clock cycles respond to environmental stimuli remains incompletely understood. Here, we show that activation of the inducible transcription factor NF-κB in response to inflammatory stimuli leads to marked inhibition of clock repressors, including the , , and genes, within the negative limb. Furthermore, activation of NF-κB relocalizes the clock components CLOCK/BMAL1 genome-wide to sites convergent with those bound by NF-κB, marked by acetylated H3K27, and enriched in RNA polymerase II. Abrogation of NF-κB during adulthood alters the expression of clock repressors, disrupts clock-controlled gene cycles, and impairs rhythmic activity behavior, revealing a role for NF-κB in both unstimulated and activated conditions. Together, these data highlight NF-κB-mediated transcriptional repression of the clock feedback limb as a cause of circadian disruption in response to inflammation.

Document type Article de périodique (Journal article)
Access type Accès libre
Publication date 2018
Language Anglais
Journal information "Genes & development" - Vol. 32, no.21-22, p. 1367-1379 (2018)
Peer reviewed yes
Publisher Cold Spring Harbor Laboratory Press ((United States) Cold Spring Harbor, NY)
issn 0890-9369
e-issn 1549-5477
Publication status Publié
Affiliation UCL - SSS/IREC/EDIN - Pôle d'endocrinologie, diabète et nutrition
MESH Subject ARNTL Transcription Factors ; Animals ; Behavior, Animal ; CLOCK Proteins ; Cell Line ; Chromatin ; Circadian Rhythm ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; NF-kappa B ; Repressor Proteins ; Transcription, Genetic
Keywords NF-κB ; Circadian ; Genomics ; High-fat diet ; Inducible transcription ; Inflammation
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Bibliographic reference Hong, Hee-Kyung ; Maury, Eléonore ; Ramsey, Kathryn Moynihan ; Perelis, Mark ; Marcheva, Biliana ; et. al. Requirement for NF-κB in maintenance of molecular and behavioral circadian rhythms in mice.. In: Genes & development, Vol. 32, no.21-22, p. 1367-1379 (2018)
Permanent URL http://hdl.handle.net/2078.1/216723