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Starvation or diabetes decreases the content but not the mRNA of 6-phosphofructo-2-kinase in rat liver.

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Crepin, K M Darville, M I Hue, Louis [UCL] Rousseau, Guy [UCL]

In rat liver, the activity of 6-phosphofructo-2-kinase (PFK-2) decreases upon starvation and in diabetes. Cyclic AMP-dependent phosphorylation of the enzyme is not sufficient to account for this decrease. PFK-2 content was therefore measured by immunotitration and relative PFK-2 mRNA levels were determined by hybridization with cDNA probes. The data are compatible with a posttranscriptional mechanism of regulation that involves decreased translational efficiency of PFK-2 mRNA and (or) increased turnover of the PFK-2 protein.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1988
Journal information "FEBS letters" - Vol. 227, no. 2, p. 136-40 (1988)
Peer reviewed yes
issn 0014-5793
Publication status Publié
Affiliation UCL - MD/BICL - Département de biochimie et de biologie cellulaire
MESH Subject Animals ; Diabetes Mellitus, Experimental - enzymology ; Kinetics ; Liver - enzymology ; Male ; Phosphofructokinase-1 - genetics - metabolism ; RNA, Messenger - genetics ; Rats ; Rats, Inbred Strains ; Starvation
Links
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Bibliographic reference Crepin, K M ; Darville, M I ; Hue, Louis ; Rousseau, Guy. Starvation or diabetes decreases the content but not the mRNA of 6-phosphofructo-2-kinase in rat liver.. In: FEBS letters, Vol. 227, no. 2, p. 136-40 (1988)
Permanent URL http://hdl.handle.net/2078.1/21643