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Oral administration of vanadate to diabetic rats restores liver 6-phosphofructo-2-kinase content and mRNA.

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Miralpeix, M Carballo, E Bartrons, R. Crepin, K Hue, Louis [UCL] Rousseau, Guy [UCL]

Vanadate and insulin were administered to diabetic (streptozotocin) rats to compare their effects on the activity and mRNA content of 6-phosphofructo-2-kinase and L-type pyruvate kinase in the liver. The activity of 6-phosphofructo-2-kinase in livers of diabetic rats was about 40% of that found in normal rats. A similar decrease was found for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase content, measured by immunoprecipitation, and for mRNA, measured by hybridization of Northern blots. Administration of vanadate to the diabetic rats led to a progressive recovery of 6-phosphofructo-2-kinase activity, and 6-phosphofructo-2-kinase/fructose- 2,6-bisphosphatase content and mRNA. This recovery, which was complete after 15 days of oral treatment, was also obtained after 60 h of insulin administration. L-type pyruvate kinase activity and mRNA were also decreased by about 70% in livers of diabetic rats. Both parameters normalized after 15 days of vanadate treatment, whereas insulin administration (60 h) raised L-pyruvate kinase mRNA three-fold above control values. Oral treatment for 15 days with vanadate can thus mimic the effect of insulin on both pyruvate kinase and 6-phosphofructo-2-kinase/fructose-2,6- bisphosphatase in livers of diabetic rats.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1992
Journal information "Diabetologia" - Vol. 35, no. 3, p. 243-8 (1992)
Peer reviewed yes
issn 0012-186X
Publication status Publié
Affiliation UCL - MD/BICL - Département de biochimie et de biologie cellulaire
MESH Subject Administration, Oral ; Animals ; Blood Glucose - metabolism ; Cytosol - enzymology ; Diabetes Mellitus, Experimental - enzymology - genetics ; Immunoassay ; Isoenzymes - genetics - metabolism ; Liver - drug effects - enzymology ; Male ; Phosphofructokinase-2 ; Phosphotransferases - genetics - metabolism ; Pyruvate Kinase - genetics - metabolism ; RNA, Messenger - genetics - metabolism ; Rats ; Rats, Inbred Strains ; Reference Values ; Vanadates - administration & dosage - pharmacology
Links
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Bibliographic reference Miralpeix, M ; Carballo, E ; Bartrons, R. ; Crepin, K ; Hue, Louis ; et. al. Oral administration of vanadate to diabetic rats restores liver 6-phosphofructo-2-kinase content and mRNA.. In: Diabetologia, Vol. 35, no. 3, p. 243-8 (1992)
Permanent URL http://hdl.handle.net/2078.1/21630